ACADVL Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	ACADVL
Locus ID	37
Kit Components	KN204428G1, ACADVL gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTGGCCGCGGCGGCGGGTAA KN204428G2, ACADVL gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTTTCGTTCCTCCCCTCCGT KN204428-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TAACAGTTCC CTCAAATTGA GAGAGCATCC CTAAATGCAG CCACCACGAG ATGCTTGTTC TAGAGTCTGA AGGCCCACCC CTTCCCTGCC AGCTGACACC CCCAGATCAG TCAGGCACTC ACAACCAATA ACCTTTACCC CATGCCAGGA GCCCCCCAAA ACTGGACAAG GCCCTTGGAA GCCAGCTTTT CCCTGGGGAG GAAAGAGGAA CCCCCCAACA TAGCCCACCT TCCCACATAC ACCAGCAGGA CTCCCTTATG AGCCAGGCTT CTCCTTAAAT AAGTCTCCCT CAAACAGCGC TGATGAACCC CCAAACCCGC CAGGCCCCCC GAACCATGGA ATATCCTAAA TTTTCCATAT CCCTACAGGT ACAAAGGGAT CACTCCAGGG GGCACCTGTG GCCAAATCCT GCATTTTCTC AGATGTGAAC CCCAAAACTA AATTAAGACC CCCAAATACA TCTTACTTCT GATGCTGCCA CTCCAGGGGC GCCAGTCCCA GATAAGGCCT ACTAACCCCT CCCCACAAAC TCATACCCTC CCCCCAGTTT ATAGCCCCCC CATCATGCTT ACCTTGGTTG TCACTATACA GAGACAGTCC AGGATGGGGG GAGGGGTGAG AGGGGAAGGA GGGGGTTGGA GAAGGGAAGG GGAGGGGAGC GTGGGAGGGA GGGGAAGGGG GCCCTAAAAG GGGCTCCCCA GTGGAGGGGA GGGGGCTTGG GGAGCACACC CCGATTCTCA GGAGGGGCGG GGGCACCGGG GGCTGGCAGC CCCGGAGTTC GGGGGCTGGG GCATGAGCTG GGGTGGGGGA GGGGAACTGG ATTTCGGGGA GCCCCGGGGT AGGGGGGGGT CTTGCCAAAC GGCCAGGGGC TAGGGGCCGT GGCGGGGGAG TGGGGTGGGG GGGTTGGAAA CGGCAGCGGC CGAGGGAGCC GTGGAGCCGA AGAGGGAAGG GGAGAGAGGA GGAGAGAGGA AGCAGGGGGA GGGAGGGAGG GAGCTAGGAG CCAGAATGGG GGGGGTGCCT TGGCAGAGTT AACTCCTTCT GTGCTGGCAG GAACCCGGAT AATGGGAAAG GAGATAGAAG CAGAAGCACA GAGGCCCCTG AGGCAAACAT GGAGACCTGG CCAGCCACCA GCGATGACAG CAAAGACGAT GAGGCTGGAT TTCATTTCTT AGAGAAGGAA GCATCTATAG TTGGGCTGGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000018, NM_001033859, NM_001270447, NM_001270448
Synonyms	ACAD6; LCACD; VLCAD
Summary	The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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