Pex5 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Pex5
Locus ID	19305
Kit Components	KN313129G1, Pex5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGGGTGGCCAGCTTCATCAG KN313129G2, Pex5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTGCTGCTGAGGCTCCCGG KN313129-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GGTCGGACGG TGCGGCCCGC GACGGCGAGC ATTGGAGCCT GGGGGCGCGG GGTCGAGGCC CGTAAGTCCC CGCCGCTGCG GTGGCCTCGT GGGACGGGGT CGAGGCTGGG GCGACGGGGC TGGGGGCGCG GGGCGCTGGG CGGGGACGGG CGCGGCCGGG GAGGGGGCTC AGGTGACACG AGCTGGGATC GGGACCCGCA GCTGGCGCCT GACCGGGGGT GTGGGTCCCG GTGTACGGTG GAAGGCGTCC CCGCTGTCCC TCGTCAGGTT AGAGTTCGGG TCTAGCCGTG TTTATGATGC GCTCCCCGTG CTCCCCAGGG GTCCAGGCCC TTTTGTAGAG CTGCTGTTGG CGGGTGGTGT CTGAGCTGCC GTCCGCTGGA GGGGAAGCCC GAGCCGAAAG CAGCGTTTCG AGTGGGAAGG CAGGACACAG CCTCCTCTTG GACTCCAGAC ACACCTCGGG GGGTGGCCCC GGGGGCCTGG CCCTTGTGGA GCCGTCGTGG GCCCATCTGC TCTAGATAGG GCCTGAGGCG TGCGTTGGGA AAGTCTGGCT TCCCGACCAC AGTTCTAATC TTCTGTCCAT CAGGGAAGCT GCTGGTCACC AGGAGAGCCT ATTTCTTCCA CCTGACAGCT GCTTTCTCTG TGTTAAAGTA ATAGCAGTCA CCATTCAGTA ACACTGGAGT GAGCCTTGTG CGGCTTTACA CAGACAAGGA CTACTATTTT ATAGACAAAG AAGCTGAGGC TTTGTGAAGG CTCTTGACTT GCCTCAGGCC ACCTAAGCAG TAGAGTTAGA GTTGGGTTGT AGCCTGCTAT TGTCTCTTTC CTTCTTATCT TAGGTTTCCA AGCCTTTGGG AGTAGGAACT GAAGACGAGG TAAACAGAGC AAGTTTCTTT TCTGTCTTTT TTTTTTTTTG GGGGGGGGGG TTGTGGTGAT TTTTTTTTTT TTTTAAATCA GTCCACTCAT ACTTCAGTCT TTGAACTCTC TTTACCAAAT GAGATTGCCT GCCAATGTTT TCGTCTACTT TTTGGGGTAT TTTTCTGGTT TCCAAATTTA CAGATAGATT TTTTTTTTTC TTTATTTATC TCCAGTTTTC GTTTCTCTGT CCAACATCAG TGGGCCCTGC CTCTGGCTGA GGCCCTCTGA TCCTAATGTT CATCCGACCA TCTCTCTGTA ATAGTTGGTG TCAGAATTCC TGCAGGACCA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001277330, NM_001277805, NM_008995, NM_175933, NM_001360570
Synonyms	AW212715; ESTM1; PTS1R; Pxr1; X83306
Summary	Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.[UniProtKB/Swiss-Prot Function]

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