Tsc22d3 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Tsc22d3
Locus ID	14605
Kit Components	KN318324G1, Tsc22d3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGGTGAGCGGCACTCGGTCT KN318324G2, Tsc22d3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGAGCTCGCAGCGGTTGGCC KN318324-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GCCGCGGAAC CCCGCGAGCC GGGAGAAGCG GGGCTGCCGC CGCCGAAGGG CGCGCGCGCT GCGGCTCTCT CTCGCTCTCT CGCTCCCTGG CCGCCCGCCC GCCCTTCCTG CCGCCTCGCA CGCAGGTCCG GCCGGAGCCA CGCGCGCACT TGGGAAAGTT GGGAGTGCGT TCGGGGTGGG GGGGGGGCGT GGGGGGGGGA CGCACGGGGA TCCTCCGGGG GAGGGGACGG GGAAGAGGGG GGCCGCCGCG GCTGCCGGAG CAGGGGGAGG AGGAGGAGGA GGAAGTGAGC CCGCAGGAGC CGCTGGGAGC TGTTTGTCCA GCTGTTTCTA TTCGCACCCC GAGCGCTCCA ACCAGAAGGG GGCCCGAGCC GACCCGCGGC GGGCACGGCT TTTTTTTTTT CTTTTTCTTT TTTTTTTTCT TTTCTTTTTT CTTTTCTTTT TTTTTTTTTT TTAAGGCGCT AATTTCTCCA ACTTTGCCCG CAGCTTGTTT TTCCGGGCAC CCTGGAGTCC CAAAAGGCTA GCTCCGCAGG TGCGCACCTG CCGGCCGCCC CCGACCTCCC TGAGCAGGCC GCCGCCGCCG CTGCCTCCAA GCCGGAGAAG AGCGGGGAGA GCCCGGGCAG CCCCTTCGTG AGCAACTTTC GGCAGCTGCA GGAGAAGCTC GTCTTCGAGA ACCTCAACAC TGACAAGCTG AACAACATAA TGCGCCAGGA TTCCATGGAG CCCGTGGTGC GCGACCCCTG CTACCTGATC AATGAGGGCA TCTGCAACCG CAACATAGAC CAGACCATGC TCTCCATTCT ACTTTTCTTC CACAGGTGGG TGGCTGGCCA GAAGGACCTC GAAAGTGGCC CTACGGGATC TTTTCCTCTG ACAGGGTGTG GGGTGGGGTG TGGGGGGGAG ACACACCAGC AAATTCCCAG GGGACATGGG GGTGGGGGGG CTCCTAGGCT GTCATGAATA GGAGACCTCT TAGCTAGCTA GAGCCTACCA GAGCTCACAG CATTCTAGCA TCTGTAAGTG TGTTTTTTGA ATGGAGCCTG GAAAGGCTTG CAAACCCCGG GGCCCACAAA ATGGGATGAC TCCATATGGG TTGAAAGTAA CTGTACGTGC ACCCTACATT TTCTGTAGTC CTTCAGACAG TTCTGCCATG GGCTTCTCTT GGAATTTGAG TTCCACCCAA CTCAGTGGGA CTGAAACTAG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001077364, NM_010286, NR_122039
Synonyms	DIP; Dsip1; Gilz; Tilz3; TSC-22R
Summary	Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities (By similarity). Isoform 1 and isoform 4 inhibit myogenic differentiation and mediate anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG.[UniProtKB/Swiss-Prot Function]

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