HSPC152 (TRMT112) (NM_016404) Human Mass Spec Standard

Specifications

Product Data
Description	TRMT112 MS Standard C13 and N15-labeled recombinant protein (NP_057488)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200036
Predicted MW	14.2 kDa
Protein Sequence	Sequence Link (showhide) >RC200036 protein sequence Red=Cloning site Green=Tags(s) MKLLTHNLLSSHVRGVGSRGFPLRLQATEVRICPVEFNPNFVARMIPKVEWSAFLEAADNLRLIQVPKGP VEGYEENEEFLRTMHHLLLEVEVIEGTLQCPESGRMFPISRGIPNMLLSEEETES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_057488
RefSeq Size	1305
RefSeq ORF	375
Synonyms	HSPC152; HSPC170; hTrm112; TRM112; TRMT11-2
Locus ID	51504
Cytogenetics	11q13.1
Summary	Acts as an activator of both rRNA/tRNA and protein methyltransferases (PubMed:25851604). Together with methyltransferase BUD23, methylates the N(7) position of a guanine in 18S rRNA (PubMed:25851604). The heterodimer with HEMK2/N6AMT1 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor (PubMed:18539146). The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species (PubMed:20308323). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production (PubMed:25851604).[UniProtKB/Swiss-Prot Function]

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