DEM1 (EXO5) (NM_022774) Human Mass Spec Standard

Specifications

Product Data
Description	DEM1 MS Standard C13 and N15-labeled recombinant protein (NP_073611)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC203370
Predicted MW	41.9 kDa
Protein Sequence	Sequence Link (showhide) >RC203370 protein sequence Red=Cloning site Green=Tags(s) MAETREEETVSAEASGFSDLSDSEFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQNWKRGLDIL SPMERFHLKYLYVTDLATQNWCELQTAYGKELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKE DAWAIKFLNILLLIPTLQSEGHIREFPVFGEVEGVLLVGVIDELHYTAKGELELAELKTRRRPMLPLEAQ KKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFSVKSLGDLMELVFLSLT LSDLPVIDILKIEYIHQETATVLGTEIVAFKEKEVRAKVQHYMAYWMGHREPQGVDVEEAWKCRTCTYAD ICEWRKGSGVLSSTLAPQVKKAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_073611
RefSeq Size	2217
RefSeq ORF	1119
Synonyms	C1orf176; DEM1; Exo V; hExo5
Locus ID	64789
Cytogenetics	1p34.2
Summary	The protein encoded by this gene is a single-stranded DNA (ssDNA)-specific exonuclease that can slide along the DNA before cutting it. However, human replication protein A binds ssDNA and restricts sliding of the encoded protein, providing a 5'-directionality to the enzyme. This protein localizes to nuclear repair loci after DNA damage. [provided by RefSeq, Nov 2016]

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