CH25H (NM_003956) Human Mass Spec Standard

Specifications

Product Data
Description	CH25H MS Standard C13 and N15-labeled recombinant protein (NP_003947)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC204716
Predicted MW	31.7 kDa
Protein Sequence	Sequence Link (showhide) >RC204716 protein sequence Red=Cloning site Green=Tags(s) MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDILCSWVPALRR YKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFF VWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWL SVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_003947
RefSeq Size	1378
RefSeq ORF	816
Synonyms	C25H
Locus ID	9023
Cytogenetics	10q23.31
Summary	This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]
Protein Families	Transmembrane
Protein Pathways	Primary bile acid biosynthesis

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