DPH4 (DNAJC24) (NM_181706) Human Mass Spec Standard

Specifications

Product Data
Description	DNAJC24 MS Standard C13 and N15-labeled recombinant protein (NP_859057)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC209088
Predicted MW	17 kDa
Protein Sequence	Sequence Link (showhide) >RC209088 protein sequence Red=Cloning site Green=Tags(s) MAVEQMPKKDWYSILGADPSANISDLKQKYQKLILMYHPDKQSTDVPAGTVEECVQKFIEIDQAWKILGN EETKREYDLQRCEDDLRNVGPVDAQVYLEEMSWNEGDHSFYLSCRCGGKYSVSKDEAEEVSLISCDTCSL IIELLHYN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_859057
RefSeq Size	3000
RefSeq ORF	444
Synonyms	DPH4; JJJ3; ZCSL3
Locus ID	120526
Cytogenetics	11p13
Summary	Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008]

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