WASH1 (WASHC1) (NM_182905) Human Mass Spec Standard
CAT#: PH309590
WASH1 MS Standard C13 and N15-labeled recombinant protein (NP_878908)
View other "WASH1" proteins (3)
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CNY 14250.00
Specifications
Product Data | |
Description | WASH1 MS Standard C13 and N15-labeled recombinant protein (NP_878908) |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence | RC209590 |
Predicted MW | 50.1 kDa |
Protein Sequence |
>RC209590 representing NM_182905
Red=Cloning site Green=Tags(s) MTPVRMQHSLAGQTYAVPFIQPDLRREEAVQQMADALQYLQKVSGDIFSRISQQVEQSRSQVQAIGEKVS LAQAKIEKIKGSKKAIKVFSSAKYPAPGRLQEYGSIFTGAQDPGLQRRPRHRIQSKHRPLDERALQEKLK DFPVCVSTKPEPEDDAEEGLGGLPSNISSVSSLLLFNTTENLYKKYVFLDPLAGAVTKTHVMLGAETEEK LFDAPLSISKREQLEQQVPENYFYVPDLGQVPEIHVPSYLPDLPGIANDLMYSADLGPGIAPSAPGTIPE LPTFHTEVAEPLKVDLQDGVLTPPPPPPPPPPAPEVLASAPPLPPSTAAPVGQGARQDDSSSSASPSVQG APREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKQQQKEQEQVRATSQGGHLMSDLFNKLVMRR KGISGKGPGAGEGPGGAFVRVSDSIPPLPPPQQPQAEEDEDDWES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-Myc/DDK |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Labeling Method | Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3 |
Reference Data | |
RefSeq | NP_878908 |
RefSeq Size | 1851 |
RefSeq ORF | 1395 |
Synonyms | FAM39E; WASH; WASH1 |
Locus ID | 100287171 |
Cytogenetics | 9p24.3 |
Summary | Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922874, PubMed:19922875, PubMed:20498093, PubMed:23452853). Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting (PubMed:20498093). Involved in endocytic trafficking of EGF (By similarity). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (PubMed:22114305). In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation (By similarity). Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (PubMed:24344185). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC405319 | WASH1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1470.00 |
|
LY405319 | Transient overexpression lysate of WAS protein family homolog 1 (WASH1) |
CNY 4840.00 |
|
TP309590 | Purified recombinant protein of Homo sapiens WAS protein family homolog 1 (WASH1), 20 µg |
CNY 2900.00 |