PFKFB1 (NM_002625) Human Mass Spec Standard

Specifications

Product Data
Description	PFKFB1 MS Standard C13 and N15-labeled recombinant protein (NP_002616)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC224599
Predicted MW	54.7 kDa
Protein Sequence	Sequence Link (showhide) >RC224599 protein sequence Red=Cloning site Green=Tags(s) MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIG TPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERR SLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEE LDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRG KQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPHEQWKALNEIDAGVCEEMTYEEIQEHYPEEF ALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLH TVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_002616
RefSeq Size	1756
RefSeq ORF	1413
Synonyms	F6PK; HL2K; PFRX
Locus ID	5207
Cytogenetics	Xp11.21
Summary	This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Protein Families	Druggable Genome
Protein Pathways	Fructose and mannose metabolism

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