PDGF Receptor beta (PDGFRB) (NM_002609) Human Over-expression Lysate
CAT＃: LC400921
PDGFRB HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
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CNY 900.00
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CNY 1999.00
CNY 2700.00
CNY 600.00
Specifications
| Product Data | |
| Description | PDGFRB HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC206377 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 123.8 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_002600 |
| Locus ID | 5159 |
| Cytogenetics | 5q32 |
| Protein Families | WB |
| Protein Pathways | Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
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| LY400921 | Transient overexpression lysate of platelet-derived growth factor receptor, beta polypeptide (PDGFRB) |
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| PH306377 | PDGFRB MS Standard C13 and N15-labeled recombinant protein (NP_002600) |
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| TP710136 | Recombinant protein of human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), residues 33-532aa, with C-terminal DDK tag, expressed in sf9, 20ug |
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| TP720649 | Purified recombinant protein of Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB) |
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| TP723757 | Purified recombinant protein of Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB / PDGF-BB) |
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其它PDGF Receptor beta产品
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
PDGFRB mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)
