VEGF-C / Flt4-L Human Protein

Specifications

Product Data
Species	Human
Expression Host	Insect
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) DPTEETIKFA AAHYNTEILK SIDNEWRKTQ CMPREVCIDV GKEFGVATNT FKPPCVSVYR CGGCCNSEGL QCMNTSTSYL SKTLFEITVP LSQGPKPVT ISFANHTSCR CMSKLHHHHHH
Predicted MW	18-24 kDa
Purity	>90% pure by SDS-PAGE and visualised by silver stain
Buffer	Presentation State: Purified State: Lyophilized protein Buffer System: PBS containing BSA (50-fold) as stabilizer
Preparation	Lyophilized protein
Protein Description	The recombinant Human VEGF-C contains 129 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Determined (i) by the ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC/VEGFR-3 cells and (ii) the VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC).
Endotoxin	< 0.1 ng per µg of VEGF-C
Reference Data
RefSeq	NP_446105
Locus ID	114111
UniProt ID	O35757
Cytogenetics	16p11
Synonyms	VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, Flt4 ligand
Summary	platelet-derived growth factor/vascular derived growth factor (PDGF/VEGF); active in angiogenesis and endothelial cell growth [RGD, Feb 2006]

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