Heat shock protein 70 / HSP70 (active) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Concentration	lot specific
Purity	>90% pure as determined by SDS-PAGE analysis.
Buffer	Presentation State: Purified State: Liquid affinity purified protein. Buffer System: Sodium Phosphate, pH 7.5 (20 mM), 150 mM NaCl, 10% Glycerol, 200 mM Imidazole.
Preparation	Liquid affinity purified protein.
Applications	ATPase Assay. Western B Control. Binding Assays. ELISA reference standard. Lipid Interaction Assays.
Protein Description	Recombinant Human Hsp70 Protein with ATPase activity, his-tagged, cloned from a Human cDNA library
Storage	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: The protein has ATPase activity at the time of manufacture of 3.3 μM phosphate liberated/hr/μg protein in a 200 μl reaction at 37°C (pH7.5) in the presence of 20 µl of 1mM ATP using a Malachite Green assay.
Reference Data
RefSeq	NP_005336
Locus ID	3303
UniProt ID	P0DMV8
Cytogenetics	6p21.33
Synonyms	HSP70.1, HSP70-1/HSP70-2, HSPA1A, HSPA1B, HSPA1
Summary	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Protein Families	ATPase Assay. Western B Control. Binding Assays. ELISA reference standard. Lipid Interaction Assays.
Protein Pathways	Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Prion diseases, Spliceosome

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