Superoxide Dismutase 1 / SOD1 (1-154) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
Predicted MW	15.9 kDa
Concentration	lot specific
Purity	>95% pure by SDS-PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris pH 7.5, 10% Glycerol
Preparation	Liquid purified protein
Applications	Protocol: Activity Assay 1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.1 mM ethylendiaminetetraacetic acid, 0.01 mM cytochrom C, 0.05 mM xanthine, 0.005 units xanthine oxidase. 2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer. 3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.
Protein Description	Recombinant SOD1 was expressed in E.coli and purified by conventional chromatography techniques.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Specific activity is >1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7. 5 at 25°C.
Endotoxin	<1.0 EU per 1 microgram of protein (determined by LAL method)
Reference Data
RefSeq	NP_000445
Locus ID	6647
UniProt ID	P00441
Cytogenetics	21q22.11
Synonyms	SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1
Summary	The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2020]
Protein Families	Protocol: Activity Assay 1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.1 mM ethylendiaminetetraacetic acid, 0.01 mM cytochrom C, 0.05 mM xanthine, 0.005 units xanthine oxidase. 2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer. 3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases

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