SQSTM1 (1-356, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MAMSYVKDDI FRIYIKEKKE CRRDHRPPCA QEAPRNMVHP NVICDGCNGP VVGTRYKCSV CPDYDLCSVC EGKGLHRGHT KLAFPSPFGH LSEGFSHSRW LRKVKHGHFG WPGWEMGPPG NWSPRPPRAG EARPGPTAES ASGPSEDPSV NFLKNVGESV AAALSPLGIE VDIDVEHGGK RSRLTPVSPE SSSTEEKSSS QPSSCCSDPS KPGGNVEGAT QSLAEQMRKI ALESEGRPEE QMESDNCSGG DDDWTHLSSK EVDPSTGELQ SLQMPESEGP SSLDPSQEGP TGLKEAALYP HLPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KHPPPL
Tag	His-Tag
Predicted MW	39.7 kDa (364aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Concentration	0.5mg/ml (determined by Bradford assay)
Purity	> 85% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant SQSTM1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Note	(Molecular weight on SDS-PAGE will appear higher).
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_001135771
Locus ID	8878
UniProt ID	Q13501
Cytogenetics	5q35.3
Synonyms	Sequestosome 1, A170, OSIL, p60, p62, p62B, PDB3, ZIP3, Sequestosome 1 EBI 3 associated protein of 60 kDa, ZIP, ZIP 3, EBI3 associated protein of 60 kDa, EBIAP, MGC127197, ORCA.M530, OSF-6, Osi, Oxidative stress induced like, Paget disease of bone 3, PDB 3, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa, Phosphotyrosine independent ligand for the Lck SH2 domain p62, PKC-zeta-interacting protein, Protein kinase C-zeta-interacting protein, SQSTM 1, STAP, STONE14, ubiquitin binding protein p62
Summary	This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Protein Families	Druggable Genome, Transcription Factors

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