RUVBL1 / TIP49A (1-456, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MKIEEVKSTT KTQRIASHSH VKGLGLDESG LAKQAASGLV GQENAREACG VIVELIKSKK MAGRAVLLAG PPGTGKTALA LAIAQELGSK VPFCPMVGSE VYSTEIKKTE VLMENFRRAI GLRIKETKEV YEGEVTELTP CETENPMGGY GKTISHVIIG LKTAKGTKQL KLDPSIFESL QKERVEAGDV IYIEANSGAV KRQGRCDTYA TEFDLEAEEY VPLPKGDVHK KKEIIQDVTL HDLDVANARP QGGQDILSMM GQLMKPKKTE ITDKLRGEIN KVVNKYIDQG IAELVPGVLF VDEVHMLDIE CFTYLHRALE SSIAPIVIFA SNRGNCVIRG TEDITSPHGI PLDLLDRVMI IRTMLYTPQE MKQIIKIRAQ TEGINISEEA LNHLGEIGTK TTLRYSVQLL TPANLLAKIN GKDSIEKEHV EEISELFYDA KSSAKILADQ QDKYMK
Tag	His-Tag
Predicted MW	52.3 kDa (476aa) confirmed by MALDI-TOF
Concentration	0.5mg/ml (determined by Bradford assay)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant RUVBL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_003698
Locus ID	8607
UniProt ID	Q9Y265
Cytogenetics	3q21.3
Synonyms	49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, INO80 complex subunit H, INO80H, NMP 238, Nuclear matrix protein 238, Pontin 52, RuvB like AAA ATPase 1, RuvB-like 1, RVB1, TAP54-alpha, TIH1, TIP49a, TIP60-associated protein 54-alpha
Summary	This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Protein Families	Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Wnt signaling pathway

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