Interleukin-12 beta / IL12B (23-328, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	Baculovirus
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) IWELKKD VYVVELDWYP DAPGEMVVLT CDTPEEDGIT WTLDQSSEVL GSGKTLTIQV KEFGDAGQYT CHKGGEVLSH SLLLLHKKED GIWSTDILKD QKEPKNKTFL RCEAKNYSGR FTCWWLTTIS TDLTFSVKSS RGSSDPQGVT CGAATLSAER VRGDNKEYEY SVECQEDSAC PAAEESLPIE VMVDAVHKLK YENYTSSFFI RDIIKPDPPK NLQLKPLKNS RQVEVSWEYP DTWSTPHSYF SLTFCVQVQG KSKREKKDRV FTDKTSATVI CRKNASISVR AQDRYYSSSW SEWASVPCS
Tag	His-Tag
Predicted MW	35.8 kDa (315aa)
Concentration	0.25mg/ml (determined by absorbance at 280nm)
Purity	> 90% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl, 0.1mM PMSF
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant human IL12P40 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_002178
Locus ID	3593
UniProt ID	P29460
Cytogenetics	5q33.3
Synonyms	Interleukin 12B, Interleukin-12 beta chain, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, interleukin 12 p40, NK cell stimulatory factor chain 2, NKSF2, CLMF2
Summary	This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus

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