IFI30 / GILT (58-232, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) NAPLVN VTLYYEALCG GCRAFLIREL FPTWLLVMEI LNVTLVPYGN AQEQNVSGRW EFKCQHGEEE CKFNKVEACV LDELDMELAF LTIVCMEEFE DMERSLPLCL QLYAPGLSPD TIMECAMGDR GMQLMHANAQ RTDALQPPHE YVPWVTVNGK PLEDQTQLLT LVCQLYQGK
Tag	His-Tag
Predicted MW	22.5 kDa (199aa) confirmed by MALDI-TOF
Concentration	1mg/ml (determined by Bradford assay)
Purity	> 85% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant Human IFI30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_006323
Locus ID	10437
UniProt ID	P13284
Cytogenetics	19p13.11
Synonyms	Gamma-interferon-inducible lysosomal thiol reductase, GILT, IFI-30, IP30
Summary	The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq, Jul 2008]
Protein Pathways	Antigen processing and presentation

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