17-beta-HSD1 / HSD17B1 (1-328, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MARTVV LITGCSSGIG LHLAVRLASD PSQSFKVYAT LRDLKTQGRL WEAARALACP PGSLETLQLD VRDSKSVAAA RERVTEGRVD VLVCNAGLGL LGPLEALGED AVASVLDVNV VGTVRMLQAF LPDMKRRGSG RVLVTGSVGG LMGLPFNDVY CASKFALEGL CESLAVLLLP FGVHLSLIEC GPVHTAFMEK VLGSPEEVLD RTDIHTFHRF YQYLAHSKQV FREAAQNPEE VAEVFLTALR APKPTLRYFT TERFLPLLRM RLDDPSGSNY VTAMHREVFG DVPAKAEAGA EAGGGAGPGA EDEAGRGAVG DPELGDPPAA PQ
Tag	His-Tag
Predicted MW	37.5 kDa (352aa) confirmed by MALDI-TOF
Concentration	1mg/ml (determined by Bradford assay)
Purity	> 90% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant human HSD17B1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_000404
Locus ID	3292
UniProt ID	P14061
Cytogenetics	17q21.2
Synonyms	Hydroxysteroid 17-beta dehydrogenase 1(approved name), 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, Estradiol 17-beta-dehydrogenase 1, Placental 17-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 28C member 1, HSD17B1, E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1
Summary	This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
Protein Families	Druggable Genome
Protein Pathways	Androgen and estrogen metabolism, Metabolic pathways

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