VEGF-C / Flt4-L Rat Protein

Specifications

Product Data
Species	Rat
Expression Host	Insect
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) DTVKLAAAHY NTEILKSIDN EWRKTQCMPR EVCIDVGKEF GAATNTFFKP PCVSVYRCGG CCNSEGLQCM NTSTGYLSKT LFEITVPLSQ GPKPVTISFA NHTSCRCMSK LDVYRQVHSI IHHHHHH
Predicted MW	15-20 kDa
Purity	>90% pure by SDS-PAGE and visualised by silver stain
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: 50 mM Acetic Acid Stabilizer: BSA
Preparation	Lyophilized purified protein
Protein Description	The recombinant Rat VEGF-C contains 115 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 15-20 kDa protein in SDS-PAGE under reducing conditions.
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Determined (i) by the ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC/VEGFR-3 cells and (ii) the VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC).
Endotoxin	< 0.1 ng per µg of VEGF-C
Reference Data
RefSeq	NP_446105
Locus ID	114111
UniProt ID	O35757
Cytogenetics	16p11
Synonyms	VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, Flt4 ligand
Summary	platelet-derived growth factor/vascular derived growth factor (PDGF/VEGF); active in angiogenesis and endothelial cell growth [RGD, Feb 2006]

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