Interleukin-3 / IL3 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF N-terminal Sequence: MAPMTQT
Predicted MW	15 kDa
Purity	>98% pure by SDS-PAGE & silver stain
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS Stabilizer: None
Preparation	Lyophilized purified protein
Protein Description	Recombinant Human IL-3 produced in E. coli is a 15.0 kDa globular protein containing 133 amino acid residues.
Note	Centrifuge vial before opening!
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Recombinant human IL-3 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1-0.5 ng/ml. Specific: 1 x 107 units/mg
Endotoxin	< 0.1 ng per µg (IEU/µg) of rh IL-3.
Reference Data
RefSeq	NP_000579
Locus ID	3562
UniProt ID	P08700
Cytogenetics	5q31.1
Synonyms	IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF
Summary	The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway

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