HSPB1 / HSP27 (1-205) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
Predicted MW	22.7 kDa (205aa) confirmed by MALDI-TOF
Concentration	1mg/ml (determined by Bradford assay)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. 20mM HEPES buffer (pH 7.4) containing 100mM KCl, 1mM DTT
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant Human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_001531
Locus ID	3315
UniProt ID	P04792
Cytogenetics	7q11.23
Synonyms	Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27.
Summary	This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
Protein Pathways	MAPK signaling pathway, VEGF signaling pathway

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