Dimt1 (NM_025447) Mouse Recombinant Protein
CAT#: TP504446
Purified recombinant protein of Mouse DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (Dimt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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Specifications
| Product Data | |
| Species | Mouse |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>MR204446 protein sequence
Red=Cloning site Green=Tags(s) MPKAKSAASSRRRDRQEQRRELKRAGGLMFNTGIGQHILKNPLIVNSIIDKAALRPTDVVLEVGPGTGNM TVKLLEKAKKVVACELDPRLVAELHKRVQGTPLASKLQVLVGDVLKSDLPFFDACVANLPYQISSPFVFK LLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIE PKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVQNTVIPEDFSIADKIQQIL TSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-MYC/DDK |
| Predicted MW | 35.3 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Reference Data | |
| RefSeq | NP_079723 |
| Locus ID | 66254 |
| UniProt ID | Q9D0D4 |
| Refseq Size | 2545 |
| Cytogenetics | 13 D2.1 |
| Refseq ORF | 939 |
| Synonyms | 1500031M22Rik; AV111347; Dimt1l |
| Summary | Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity.[UniProtKB/Swiss-Prot Function] |
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