Pcif1 (NM_146129) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR210164 protein sequence Red=Cloning site Green=Tags(s) MANENHGSPREGASLLSHSPGTSSQSQPCSPKPVRLVQDLPEELVHAGWEKCWSRRESRPYYFNRFTNQS LWEMPVLGQHDVLSDPLGLNATPLPQDSSLVETPPVENKSRKRQLSEEQPSGNGVKKPKIEIPVTPTSQS VPSSPSIPGTPTLKIWGSSTEDKQAALLRPTEVYWDLDIQTNAVIKHRGPSEVLPPHPDVELLRSQLILK LRQHYRELCQQREGIEPPRESFNRWMLERKVVDKGCDPLLPSNCEPVVSPSMFREIMNDIPIRLSRIKFR EEAKRLLFKYAEAARRLIESRSASPDSRKVVKWNVEDTFSWLRKEHSASKEDYMDRLEHLRRQCGPHVSA AAKDSVEGICSKIYHISLEYVKRIREKHLAVLKENNIPEEVEASELEPRLVYCYPVRLAVSAPPMPSVEM HVENSVVCIRYKGEMVKVSRSYFSKLWLLYRYSCVDDSAFERFLPRVWCLLRRYQMMFGVGLYEGTGLQG SLPVHVFETLHRLFGVSFECFASPLNCYFRQYCSAFPDTDGYFGSRGPCLDFTPLSGSFEANPPFCEELM DAMVSHFEKLLESSAEPLSFIVFIPEWREPPTPALTRMEQSRFKRHQLVLPAFEHEYRSGSQHICKKEEM HYKAVHNTAVLFLQNGPGFAKWGPTPERLQELTAAYKQSGRSHGSSSSSSSSSSSSEAKDRDSGREQGPS REPHPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	80.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_666241
Locus ID	228866
UniProt ID	P59114
Refseq Size	2752
Cytogenetics	2 H3
Refseq ORF	2118
Synonyms	2310022K11Rik; C20orf67; F730014I05Rik
Summary	Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally. N6-methylation of m6A(m) promotes the translation of capped mRNAs.[UniProtKB/Swiss-Prot Function]

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