Nckipsd (NM_030729) Mouse Recombinant Protein
CAT#: TP510218
Purified recombinant protein of Mouse NCK interacting protein with SH3 domain (Nckipsd), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR210218 representing NM_030729
Red=Cloning site Green=Tags(s) MYRALYAFRSAEPNAMAFAAGETFLVLERSSTHWWLAARARSGETGYVPPAYLHRLQGMEQDVLQAIDRA IEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGTSASSATVMTPSTSDHHLDAAVSRQPNGVCRT GFERQHSLPSSEHLGTDGALYQVPPQPRRAAPTTPPPPVKRRDREALVISGSGGRTAIPSGGSSVSSGSS ASSTSMDTLYTGSSPSELGPSCSPTPPPVPRRGAHTTVSQPQPSPSKAPSPEPPTEEVAAETNSTPDDLE AQDALSPETTEEKAAAETVVPRTIGAELMELVRRNTGLSHELCRVAIGVVVGHIQATVPASSPIMEQVLL SLVEGKDLSTALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEDVIRCYLEELLHILTDADPEV CKKMCKRSDFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDH QKLCYSALVLAMVFSMGEAVPYAHYEHLGTPFAQFLLSIVEDGLPMDTTEQLPDLCMNLLLALNLHLTAP EQNVIMAALSRHTNVKIFSEKLLLLLNRGDDPVRIFRHEPQPPHSVLKFLQDVFSSSATAAIFYHTDMMA LIDITVRQIADLSPGDKLRMEYLSLMHAVVRSTPYLQHRHRLSDLQATLRRILTEEEASPQCQMDRMIVQ EMYKEFPDLGEVPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 78.6 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_109654 |
Locus ID | 80987 |
UniProt ID | Q9ESJ4 |
Refseq Size | 2870 |
Cytogenetics | 9 F2 |
Refseq ORF | 2142 |
Synonyms | AF3P21; DIP1; ORF1; SPIN90; Wasbp; WASLBP; WISH |
Summary | Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1) (By similarity). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.[UniProtKB/Swiss-Prot Function] |
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