Recombinant CXCL5 (C-X-C motif chemokine 5), Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) RELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN with polyhistidine tag at the N-terminus.
Tag	His Tag (N-term)
Predicted MW	The protein has a calculated MW of 8.51 kDa. The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED₅₀ for this effect is <10 ng/mL.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	P42830
Synonyms	Epithelial Neutrophil Activating Peptide-78, ENA-78
Summary	C-X-C motif chemokine 5 (CXCL5) also named epithelial-derived neutrophil-activating peptide 78 (ENA-78), which is a chemokine of the intercrine alpha family. CXCL5 is a 8kDa protein containing 70 amino acid residues. CXCL5 is stimulated by the IL-1 or TNFα during inflammation which produced by the eosinophils and CXCL5 is inhibited by the IFNγ. CXCL5 promotes the formation of blood vessels and angiogenesis by binding the cell receptor CXCR2.

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