BMP2 (NM_001200) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region (Gln283-Arg396) of human BMP2
Tag	Tag Free
Predicted MW	13 kDa
Concentration	Resuspend the protein to the desired concentration in proper buffer.
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a sterile solution containing 10 mM sodium citrate, pH 3.5
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Measured?by?its?ability?to?induce?alkaline?phosphatase?production in?ATDC5?cells. ED50 for this effect is typically 36 - 55 ng/ml. Co-immunoprecipitation (PMID: 28009989)
Endotoxin	< 0.1 EU per 1 µg of the protein by the LAL
Reference Data
RefSeq	NP_001191
Locus ID	650
UniProt ID	P12643
Refseq Size	1547
Cytogenetics	20p12.3
Refseq ORF	1188
Synonyms	BDA2; BMP2A; SSFSC; SSFSC1
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
Protein Pathways	Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway

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