TEV protease Tobacco etch virus Recombinant Protein

Specifications

Product Data
Species	Tobacco etch virus
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MHHHHHHMGE SLFKGPRDYN PISSTICHLT NESDGHTTSL YGIGFGPFII TNKHLFRRNN GTLLVQSLHG VFKVKNTTTL QQHLIDGRDM IIIRMPKDFP PFPQKLKFRE PQREERICLV TTNFQTKSMS SMVSDTSCTF PSSDGIFWKH WIQTKDGQCG SPLVSTRDGF IVGIHSASNF TNTNNYFTSV PKNFMELLTN QEAQQWVSGW RLNADSVLWG GHKVFMVKPE EPFQPVKEAT QLMN
Tag	N-HIS
Predicted MW	27.8KDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 5 mM DTT, 50% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -20°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	1 unit is defined as the amount of enzyme required to cleave 3 µg of a substrate protein to 85% completion in 1 hours at 30°C.
Endotoxin	< 1 EU per 1 µg of the protein by the LAL
Reference Data

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