splicing factor 1 (SF1) Human shRNA Plasmid Kit (Locus ID 7536)

Specifications

Product Data
Product Name	splicing factor 1 (SF1) Human shRNA Plasmid Kit (Locus ID 7536)
Locus ID	7536
UniProt ID	Q15637
Synonyms	BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162
Vector	pRS
Format	Retroviral plasmids
Kit Components	SF1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 7536). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001178030, NM_001178031, NM_004630, NM_201995, NM_201997, NM_201998, NR_033649, NR_033650, NM_001346363, NM_001346364, NM_001346409, NM_001346410, NM_201998.1, NM_201998.2, NM_004630.1, NM_004630.2, NM_004630.3, NM_201995.1, NM_201995.2, NM_201997.1, NM_201997.2, NM_001178031.1, NM_001178031.2, NM_001178030.1, BC020217, BC020217.1, BC038446, BC000773, BC008080, BC008724, BC011657, BC032676, BC034451, BC069273, NM_201995.3, NM_001178031.3, NM_004630.4, NM_001178030.2, NM_201998.3
Summary	This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.