PYHIN1 Human shRNA Plasmid Kit (Locus ID 149628)

Specifications

Product Data
Product Name	PYHIN1 Human shRNA Plasmid Kit (Locus ID 149628)
Locus ID	149628
UniProt ID	Q6K0P9
Synonyms	IFIX
Vector	pRS
Format	Retroviral plasmids
Kit Components	PYHIN1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 149628). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_152501, NM_198928, NM_198929, NM_198930, NM_198929.1, NM_198929.2, NM_198929.3, NM_198929.4, NM_198928.1, NM_198928.2, NM_198928.3, NM_198928.4, NM_198930.1, NM_198930.2, NM_198930.3, NM_152501.1, NM_152501.2, NM_152501.3, NM_152501.4, BC090944, BC090944.1, BC020822, BC073133, BC114470, BC139741, NM_198930.4, NM_198928.5, NM_152501.5, NM_198929.5
Summary	The protein encoded by this gene belongs to the HIN-200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-termini. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis. Downregulation of this gene is associated with breast cancer. This protein acts as a tumor suppressor by promoting ubiquitination and subsequent degradation of MDM2, which leads to stabilization of p53/TP53. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.