pT7-Cas9

CAT＃: GE100014

pT7-Cas9 vector in which Cas9 is under T7 for IVT to produce Cas9 mRNA

Datasheet
SDS
Vector Sequence

CNY 3710.00

货期*
现货
规格
    • 10 ug
Cited in 4 publications.

Product images

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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暂停销售

Specifications

Product Data
Function CRISPR/Cas9 T7 Vectors
Features
  • T7 driven Cas9 expression vector
    1. Cas9 mRNA can be produced via in vitro transcription.
    2. For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven gRNA can be produced from a separate vector, SKU GE100025.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (4)

The use of this Vectors has been cited in the following citations:
Two CRISPR/Cas9-mediated methods for targeting complex insertions, deletions, or replacements in mouse ,Pineault, K;Novoa, A;Lozovska, A;Wellik, D;Mallo, M;, MethodsX ,PubMed ID 31667107 [pT7-Cas9]
PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction ,Zhou, W;Ma, D;Sun, AX;Tran, HD;Ma, DL;Singh, BK;Zhou, J;Zhang, J;Wang, D;Zhao, Y;Yen, PM;Goh, E;Tan, EK;, Hum. Mol. Genet. ,PubMed ID 30496485 [pT7-Cas9]
KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system ,Yang, L;Song, L;Liu, X;Bai, L;Li, G;, EMBO reports ,PubMed ID 30389724 [pT7-Cas9]
Fine Tuning of Histone Demethylase KDM6A/B Improves the Development of Nuclear Transfer Embryo ,Yang, L;Song, L;Liu, X;Bai, L;Li, G;, bioRxiv [pT7-Cas9]

Documents

Product Manuals
FAQs
SDS
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