CRISPRa SAM Vector Kit, CRISPR/Cas9 Gene Activation

CAT＃: GE100057

CRISPRa SAM vector kit containing pCas-Guide-CRISPRa (GE100055), pCRISPRa-Enhancer (GE100056) and pCas-Guide-CRISPRa-Scramble (GE100058)

Datasheet
SDS

CNY 10,545.00

货期*
2周
规格
    • 1 kit
Cited in 4 publications.

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TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
    • 500 pmol

CNY 1,020.00

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CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Specifications

Product Data
Function CRISPRa SAM vector kit
Features
  • Robust CRISPR gene activation system
  • Synergistic activation by three activation domains, VP64, p65 and HSF1
  • Two vector system, dCas9-VP64-gRNA(MS2) (SKU GE100055) and MS2-p65-HSF1 (SKU GE100056)
  • Scramble gRNA control (SKU GE100058) is included
  • CF3 primer can be used to sequence target sequence after cloning
  • Complement to gene overexpression with cDNA clones
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (4)

The use of this Vectors has been cited in the following citations:
Ovotransferrin Exhibits Osteogenic Activity Partially via Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Activation in MC3T3-E1 Cells. ,null, Journal of agricultural and food chemistry ,PubMed ID 32786820
Rapid identification of human mast cell degranulation regulators using functional genomics coupled to high-resolution confocal microscopy ,null, Nature protocols ,PubMed ID 32060492
Mesenchymal Stem Cell Engineering. ,null, Methods in molecular biology (Clifton, N.J.) ,PubMed ID 30244462
PGE2/EP3/SRC signaling induces EGFR nuclear translocation and growth through EGFR ligands release in lung adenocarcinoma cells ,null, Oncotarget ,PubMed ID 28415726

Documents

Product Manuals
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SDS
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