pAAVS1-EF1a-tGFP-BSD-DNR

Specifications

Product Data
Function	AAVS1 donor vector
Features	Donor vector for targeted transgene insertion into human AAVS1 site Contains left and right AAVS1 homologous sequences (550 bp each side) Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones The expression of the target gene will be under EF1a promoter, a constitutive promoter as an alternative of CMV promoter. the latter could be silenced in certain cellular environment Blasticidin mammalian selection under PGK promoter Targeted insertion when cotransfected with pCas-Guide-AAVS1 (SKU GE100023)

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