pLenti-EF1a-C-Myc-DDK-IRES-Puro Lentiviral Gene Expression Vector

Specifications

Product Data
C-Tag	Myc-DDK
E. coli Selection	Chloramphenicol (34 ug/mL)
Cell Selection	Puromycin
Features	This Lenti vector is a third generation of lentiviral vector. All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment. The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags. IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided EF51 Primer can be used to sequence the target sequence after cloning. IRES100 Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

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