pLenti-EF1a-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector

Specifications

Product Data
C-Tag	Myc-DDK
E. coli Selection	Chloramphenicol (34 ug/mL)
Cell Selection	Puromycin
Features	This Lenti vector is a third generation lentiviral vector. The ORF cloned in this vector can be expressed as a tagged fusion protein with C-terminal Myc-DDK tag. The expression of the cloned ORF is driven by EF1a promoter, a constitutive promoter. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphenicol (34ug/ml) All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. EF51 Primer can be used to sequence the target sequence after cloning. P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

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