pCMV6-AN-3DDK Mammalian Expression Vector

Specifications

Product Data
N-Tag	3DDK
E. coli Selection	Ampicillin (100 ug/mL)
Cell Selection	Neomycin
Features	ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins with 3 tandem DDK-tags on the C-terminal ends, resulting in 100X higher detection sensitivity than 1XDDK tag The 3DDK tag will increase the affinity of the fusion protein to anti-DDK tags and facilitate detection. This is especially useful for proteins of low expression level. If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal 3DDK-tagging vector, PS100057. V2_F Primer can be used to sequence the target sequence after cloning. XL39_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites

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