Mitochondria Mouse Monoclonal Antibody [Clone ID: SPM198]

CAT#: AM33287PU-S

Mitochondria mouse monoclonal antibody, clone SPM198, Purified

Size: 20 ug 100 ug



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CNY 5830.00


货期*
4周

规格
    • 100 ug

Cited in 1 publication.

Product images

Specifications

Product Data
Clone Name SPM198
Applications IF, IHC, WB
Recommend Dilution Western Blot: 0.25-0.5 µg/ml.
Immunofluorescence
: 0.5-1 µg/ml.
Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control: HeLa or HepG2 cells, Hepatic carcinoma.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Semi-purified mitochondrial preparation.
Specificity This Monoclonal recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Cellular Localization: Mitochondria in cytoplasm.
Negative Species: Mouse and Rat.
Isotype IgG1
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
DO NOT FREEZE!
Predicted Protein Size 60 kDa
Background Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998).
Synonyms Mitochondrial Marker
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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