Mitochondria Mouse Monoclonal Antibody [Clone ID: SPM198]

Specifications

Product Data
Clone Name	SPM198
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 0.25-0.5 µg/ml. Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: HeLa or HepG2 cells, Hepatic carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Semi-purified mitochondrial preparation.
Specificity	This Monoclonal recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Cellular Localization: Mitochondria in cytoplasm. Negative Species: Mouse and Rat.
Isotype	IgG1
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	60 kDa
Background	Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998).
Synonyms	Mitochondrial Marker
Reference Data

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