MHC Class I (RT1Aa) Mouse Monoclonal Antibody [Clone ID: OX-18]

Specifications

Product Data
Clone Name	OX-18
Applications	FC, IHC, IP
Recommend Dilution	Flow cytometry: 1:50-1:100, use 10 µl of the suggested working dilution to label 10e6 cells. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable on paraffin embedded sections. ELISA. Immunoprecipitation.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat spleen cell glycoprotein
Specificity	This antibody recognises a monomorphic determinant of rat MHC Class I (RT-1A). However, quantitative measurements suggest that not all of the class I molecules are recognised. MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules (see Ref. 1). This product is routinely tested in flow cytometry on rat splenocytes.
Isotype	IgG1
Formulation	PBS, pH 7.4, containing 0.09% sodium azide as preservative State: Purified State: Liquid
Concentration	lot specific
Purification	Protein G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Database Link	UniProt ID P16391
Background	MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Reference Data

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