MHC Class I (RT1Aa) Mouse Monoclonal Antibody [Clone ID: OX-18]

Specifications

Product Data
Clone Name	OX-18
Applications	FC, IHC, IP
Recommend Dilution	Flow cytometry: use 10 µl of 1/50 - 1/100 diluted antibody to label 10e6 cells in 100 µl. (This product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections: Acetone fixation is recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable for immunohistochemistry on paraffin sections. ELISA. Immunoprecipitation.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat spleen glycoproteins
Specificity	This antibody recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, qualitative measurements suggest that not all of the class I molecules are recognized.
Isotype	IgG1
Formulation	PBS, pH 7.4, containing 0.09% sodium azide State: Purified State: Liquid IgG fraction
Concentration	lot specific
Purification	Protein G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P16391
Background	MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Note	MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.