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Nlrp10 (NM_175532) Mouse Tagged ORF Clone
CAT＃: MR223786
- TrueORF®
Nlrp10 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 10 (Nlrp10)
ORF Plasmid: tGFP
"NM_175532" in other vectors (4)
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CNY 7,408.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6430548I20Rik; Nalp10; Napl10; PAN5; Pynod |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223786 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGGCACGGGCCAACAGCCCCCAGGAGGCACTGCTTTGGGCCTTGAATGACCTTGAGGAGAACA GTTTCAAGACGCTGAAGTTCCACTTACGAGATGTGACCCAATTTCATCTGGCCCGAGGGGAACTGGAGAG TCTGAGTCAGGTGGACCTGGCATCAAAACTGATTTCAATGTACGGAGCACAGGAAGCCGTGAGAGTGGTG AGCAGGAGCTTGCTGGCCATGAACTTGATGGAGCTTGTAGACTACCTCAACCAGGTTTGTCTGAATGATT ACAGAGAAATCTACCGGGAGCATGTGCGCTGCTTGGAGGAGAGGCAAGATTGGGGTGTTAATAGCAGCCA CAATAAGCTGCTCCTGATGGCCACATCCAGTTCAGGGGGTCGCAGATCACCTTCCTGTTCTGACCTGGAA CAGGAGTTGGATCCTGTCGATGTGGAGACTTTATTTGCTCCGGAGGCGGAGTCCTACTCAACCCCGCCCA TAGTGGTGATGCAAGGATCTGCGGGCACTGGAAAGACAACCCTGGTCAAAAAACTGGTGCAGGACTGGTC CAAAGGGAAGCTGTACCCAGGCCAGTTTGATTATGTTTTCTACGTGAGCTGCAGAGAAGTGGTCCTGTTA CCCAAGTGTGACCTGCCCAACCTCATCTGCTGGTGTTGTGGAGATGATCAAGCCCCAGTCACAGAGATTC TGCGGCAGCCTGGGCGGCTCCTGTTCATCCTGGATGGCTATGATGAGTTACAGAAGTCCAGTCGTGCCGA GTGCGTACTTCACATTCTGATGAGGAGAAGGGAGGTACCATGCTCCCTTCTCATCACCACGCGGCCTCCA GCTTTGCAGAGTCTGGAACCCATGTTGGGTGAACGGCGACATGTCCTTGTCTTAGGCTTCTCTGAGGAGG AGAGGGAAACATATTTCAGCTCCTGTTTTACGGATAAGGAGCAATTGAAAAATGCCCTTGAGTTTGTGCA AAACAATGCTGTTCTCTATAAAGCATGCCAGGTTCCAGGCATTTGCTGGGTGGTCTGCTCCTGGCTGAAG AAGAAGATGGCGAGAGGTCAGGAAGTCTCAGAGACACCTAGCAACAGTACAGACATCTTCACTGCTTATG TGTCCACCTTTCTGCCCACTGATGGCAATGGAGACAGCTCTGAGCTTACCAGGCACAAGGTACTGAAGAG TCTGTGCTCCTTGGCTGCGGAGGGGATGCGGCACCAGAGGCTGCTATTTGAAGAAGAGGTCCTCAGAAAG CATGGCTTAGACGGCCCCAGCCTTACTGCTTTCCTGAACTGCATCGACTACCGAGCGGGGCTTGGCATCA AGAAATTCTACAGCTTTCGCCACATCAGCTTCCAGGAATTTTTCTATGCCATGTCCTTCCTTGTGAAGGA GGACCAGAGTCAGCAGGGAGAGGCCACACACAAAGAAGTGGCAAAGCTGGTGGATCCAGAGAACCATGAA GAGGTGACTCTCAGTTTGCAGTTCTTATTCGACATGTTGAAAACAGAGGGCACCTTGAGCTTGGGGCTGA AGTTCTGCTTCAGAATCGCTCCTTCGGTAAGGCAGGATCTGAAGCATTTTAAAGAGCAAATAGAAGCCAT AAAGTACAAAAGGTCATGGGATCTGGAATTCTCTCTCTATGATTCTAAGATAAAGAAGCTCACACAAGGT ATCCAGATGAAAGATGTCATTCTCAATGTGCAACATTTGGATGAAAAGAAATCTGACAAGAAGAAGTCAG TTTCAGTGACAAGCAGCTTTAGTAGCGGGAAGGTACAAAGTCCATTCTTGGGAAATGATAAAAGCACCAG GAAACAAAAGAAGGCATCTAATGGGAAAAGCAGAGGGGCAGAGGAACCAGCCCCGGGGGTTAGAAACCGT AGGCTGGCAAGCAGAGAAAAGGGTCATATGGAGATGAATGATAAGGAGGATGGTGGGGTGGAGGAACAGG AGGATGAGGAAGGACAGACACTTAAAAAGGATGGAGAGATGATAGATAAGATGAATGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223786 protein sequence
Red=Cloning site Green=Tags(s) MALARANSPQEALLWALNDLEENSFKTLKFHLRDVTQFHLARGELESLSQVDLASKLISMYGAQEAVRVV SRSLLAMNLMELVDYLNQVCLNDYREIYREHVRCLEERQDWGVNSSHNKLLLMATSSSGGRRSPSCSDLE QELDPVDVETLFAPEAESYSTPPIVVMQGSAGTGKTTLVKKLVQDWSKGKLYPGQFDYVFYVSCREVVLL PKCDLPNLICWCCGDDQAPVTEILRQPGRLLFILDGYDELQKSSRAECVLHILMRRREVPCSLLITTRPP ALQSLEPMLGERRHVLVLGFSEEERETYFSSCFTDKEQLKNALEFVQNNAVLYKACQVPGICWVVCSWLK KKMARGQEVSETPSNSTDIFTAYVSTFLPTDGNGDSSELTRHKVLKSLCSLAAEGMRHQRLLFEEEVLRK HGLDGPSLTAFLNCIDYRAGLGIKKFYSFRHISFQEFFYAMSFLVKEDQSQQGEATHKEVAKLVDPENHE EVTLSLQFLFDMLKTEGTLSLGLKFCFRIAPSVRQDLKHFKEQIEAIKYKRSWDLEFSLYDSKIKKLTQG IQMKDVILNVQHLDEKKSDKKKSVSVTSSFSSGKVQSPFLGNDKSTRKQKKASNGKSRGAEEPAPGVRNR RLASREKGHMEMNDKEDGGVEEQEDEEGQTLKKDGEMIDKMNG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175532 |
| ORF Size | 2022 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175532.3, NP_780741.1 |
| RefSeq Size | 4483 bp |
| RefSeq ORF | 2022 bp |
| Locus ID | 244202 |
| UniProt ID | Q8CCN1 |
| MW | 76.4 kDa |
| Gene Summary | Inhibits autoprocessing of CASP1, CASP1-dependent IL1B secretion, PYCARD aggregation and PYCARD-mediated apoptosis but not apoptosis induced by FAS or BID (By similarity). Displays anti-inflammatory activity (By similarity). Required for immunity against C.albicans infection (PubMed:23071280). Involved in the innate immune response by contributing to proinflammatory cytokine release in response to invasive bacterial infection (By similarity). Contributes to T-cell-mediated inflammatory responses in the skin (PubMed:27221772). Plays a role in protection against periodontitis through its involvement in induction of IL1A via ERK activation in oral epithelial cells infected with periodontal pathogens (By similarity). Exhibits both ATPase and GTPase activities (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220382 | Nlrp10 (untagged) - Mouse NLR family, pyrin domain containing 10 (Nlrp10), (10ug) |
CNY 8,650.00 |
|
| MG223786 | Nlrp10 (tGFP-tagged) - Mouse NLR family pyrin domain containing 10 (Nlrp10), (10ug) |
CNY 9,008.00 |
|
| MR223786L3 | Lenti ORF clone of Nlrp10 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 10 (Nlrp10) |
CNY 7,890.00 |
|
| MR223786L4 | Lenti ORF clone of Nlrp10 (mGFP-tagged) - Mouse NLR family, pyrin domain containing 10 (Nlrp10) |
CNY 7,890.00 |
