Ciita (NM_001302618) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C2t; C2ta; EG669998; Gm9475; Mhc2ta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231631 representing NM_001302618 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCACTTCCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAGCCCAGGCAGGTGC GGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCTCTCACGGGAATACCACTGTGCCTTGCTGCATGA GCCTGATGGTGATGCCCTGGCCCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTGACT TTCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAGGGGCACCAGCTACAGGGACCATG GAGACCATAGTCTGTGTGCCACCATGGATCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAG TGATGCCGACCCCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGAGGAGGAGATCGAA CTCAGCTCAGAGCCAGACACAGATACCATCAACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAAC TGGATGAAGAGACCCGGGAGGCCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCA GCTCGAGGGCCTGAGCAAGGACCTCTTCATAGAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATA GAGATCCCTGTAGAAGCAGGACAGAAGCCTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAA AGCACAGGAAGCTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCACTGGGCACATCCA GATCTTCACCACTCTGCCCCAGGGACTCTGGCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTA ATCTACCACGGTGAGATGCCCCAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCC CCGAGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCC CGAACCTGCGCTGACCTCCCGTGTAAATGAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCC GAGTCTTCCATCAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCCTACAGGACAAAT ACAAGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAG AGGCAGCAACAAGAGCCAGGAAAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGT GGTCTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGC TGGGCAAGGCTGGCCAGGGAAAGAGCCACTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTT GCTACAATATGACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGACACCTACCACCTG CGGGATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTGA GGCAGCCAGACCGTGTTCTGCTCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCA CGGACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGG AAGCTACTGCGAGGCTGCACACTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCA AGGCAGATGCCATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACATGAGGCACTACTT TGAGAACTCAGGGACAGCGGGGAACCAAGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGC AGCTATAGTCACAGCCCTGTTGTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCA CAGAGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAG TCCTCCCGGAGCCTTAGTCGAGCTGGCCAAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTG CAAGAAACCCGGTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGATGCAGCAGACCC TGGAGACCACGGAGGCTCAACTGGCCTTCTCCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCT GGCACAGTGCAATGAAATCAAAGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGA CCCTATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACT GCCTGGGAGCTCTGGTGGAGCCTGCAGTGGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTA CCTGAAGCGCCTGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCACTGTGCCCACGAG ACACAGCAACCTGGGATATGGGAGCATGTTGCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACCC GGCTCACACCCCCAGATGTGTATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGA CCTTCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTC AGGGCCTCCTTGAGTGATACAATGGCATTATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCC AGGCGGCAGAGGAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGTGGAAGACCTGGA TCGTCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCGG GACCTTAAGAAGCTAGAGTTTGCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGA TCCTTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAA GGGTGTGTCGAAGCTCTCAGCCACCTTCCCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAAC AACATCACTGATGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGTCCCTCCTAAGGC TGAGCTTGTACAATAACTGCATCTGTGACAAAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGGT GTCCCTGCGTGTGATGGATGTCCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGC CTTCAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAAC ACCTGCAGCAGCTGGATGCCAGGATCAGTCTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231631 representing NM_001302618 Red=Cloning site Green=Tags(s) MNHFQAILAQVQTLLSSQKPRQVRALLDGLLEEELLSREYHCALLHEPDGDALARKISLTLLEKGDLDLT FLSWVCNSLQAPTVERGTSYRDHGDHSLCATMDLGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEEIE LSSEPDTDTINCDQFSKLLQDMELDEETREAYANIAELDQYVFQDTQLEGLSKDLFIEHIGAEEGFGENI EIPVEAGQKPQKRRFPEEHAMDSKHRKLVPTSRTSLNYLDLPTGHIQIFTTLPQGLWQISGAGTGLSSVL IYHGEMPQVNQVLPSSSLSIPSLPESPDRPGSTSPFTPSAADLPSMPEPALTSRVNETEDTSPSPCQEGP ESSIKLPKWPEAVERFQHSLQDKYKALPQSPRGPLVAVELVRARLERGSNKSQERELATPDWTERQLAHG GLAEVLQVVSDCRRPGETQVVAVLGKAGQGKSHWARTVSHTWACGQLLQYDFVFYVPCHCLDRPGDTYHL RDLLCPPSLQPLAMDDEVLDYIVRQPDRVLLILDAFEELEAQDGLLHGPCGSLSPEPCSLRGLLAGIFQR KLLRGCTLLLTARPRGRLAQSLSKADAIFEVPSFSTKQAKTYMRHYFENSGTAGNQDKALGLLEGQPLLC SYSHSPVVCRAVCQLSKALLEQGTEAQLPCTLTGLYVSLLGPAAQNSPPGALVELAKLAWELGRRHQSTL QETRFSSVEVKTWAVTQGLMQQTLETTEAQLAFSSFLLQCFLGAVWLAQCNEIKDKELPQYLALTPRKKR PYDNWLEGVPRFLAGLVFQPRAHCLGALVEPAVAAVADRKQKVLTRYLKRLKLGTLRAGRLLELLHCAHE TQQPGIWEHVAHQLPGHLSFLGTRLTPPDVYVLGRALETASQDFSLDLRQTGVEPSGLGNLVGLSCVTSF RASLSDTMALWESLQQQGEAQLLQAAEEKFTIEPFKAKSPKDVEDLDRLVQTQRLRNPSEDAAKDLPAIR DLKKLEFALGPILGPQAFPTLAKILPAFSSLQHLDLDSLSENKIGDKGVSKLSATFPQLKALETLNLSQN NITDVGACKLAEALPALAKSLLRLSLYNNCICDKGAKSLAQVLPDMVSLRVMDVQFNKFTAAGAQQLASS LQKCPQVETLAMWTPTIPFGVQEHLQQLDARISLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302618
ORF Size	3465 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302618.1, NP_001289547.1
RefSeq Size	5438 bp
RefSeq ORF	3468 bp
Locus ID	12265
UniProt ID	P79621
MW	128 kDa
Gene Summary	This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

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