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EIF2A (NM_032025) Human Tagged ORF Clone
CAT＃: RC204303
EIF2A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A)
ORF Plasmid: tGFP
"NM_032025" in other vectors (6)
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CNY 4,360.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDA02; EIF-2A; MST089; MSTP004; MSTP089 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAATGGACCACCAC ATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAAGTCTGTATCTTTAGTAAGGA TGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAATATTATCAGTGTCACTAACAAGGGACTACTG CACTCCTTCGACCTCCTGAAGGCAGTTTGCCTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGC AGCCTTACACTACTTCTAAAGATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGG GACATGTTTGAAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACAATTGCAAATA AATTGCATTTGCAAAAAATTAATGACTTTGTATTATCACCTGGACCCCAACCATACAAGGTGGCTGTCTA TGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTATATCAGTACCCCAACTTTGCTGGACCTCAT GCAGCTTTAGCTAATAAAAGTTTCTTTAAGGCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTG CTGTGTTGGTAATAGCTAGCACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACA CTACATTGCAACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACAATTTTCAACT TGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCCTACTATAGCCCTCATGGACA TATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAAATGGAAGTGTGGGATGTGAAAAACTACAAA CTTATTTCTAAACCGGTGGCTTCTGATTCTACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAA CAGCTACATGTGCTCCCAGGTTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTT GCACAAGTATGATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCTAAAGTTGCAA CAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAATTGCATGAAGAGGAACCACC TCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTATCAAAAACAGCTCTTAAAAATCAAAGGAAG CATGAAGCTAAGAAAGCTGCAAAGCAGGAAGCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTG CCCCACAGAGCACACCACGAAACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAAT CAAGAACCTAAAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGATTTGAAATTGG GTATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204303 protein sequence
Red=Cloning site Green=Tags(s) MAPSTPLLTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIFSKDGTLFAWGNGEKVNIISVTNKGLL HSFDLLKAVCLEFSPKNTVLATWQPYTTSKDGTAGIPNLQLYDVKTGTCLKSFIQKKMQNWCPSWSEDET LCARNVNNEVHFFENNNFNTIANKLHLQKINDFVLSPGPQPYKVAVYVPGSKGAPSFVRLYQYPNFAGPH AALANKSFFKADKVTMLWNKKATAVLVIASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVV WNSSSTEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYK LISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSILHKYDVPSNAELWQVSWQPFLDGI FPAKTITYQAVPSEVPNEEPKVATAYRPPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRK HEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQAATGKQL EKNQLEKIQKETALLQELEDLKLGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032025 |
| ORF Size | 1755 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032025.5 |
| RefSeq Size | 3894 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 83939 |
| UniProt ID | Q9BY44 |
| MW | 65 kDa |
| Gene Summary | This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204303L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC204303L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), mGFP tagged |
CNY 6,460.00 |
|
| RC204303L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC204303L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), mGFP tagged |
CNY 6,460.00 |
|
| RG204303 | EIF2A (tGFP-tagged) - Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A) |
CNY 5,040.00 |
|
| SC316786 | EIF2A (untagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A) |
CNY 4,360.00 |
