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EIF2A (NM_032025) Human Untagged Clone
CAT＃: SC316786
EIF2A (untagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A)
CNY 4,360.00
CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDA02; EIF-2A; MST089; MSTP004; MSTP089 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_032025 edited
ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAAT GGACCACCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAA GTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAAT ATTATCAGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGC CTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACAGTACTTCTAAA GATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTG AAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACA ATTGCAAATAAATTGCATTTGCAAAAAATTAATGATTTTGTATTATCACCTGGACCCCAA CCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTA TATCAGTACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAG GCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGTTGGTAATAGCTAGC ACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACACTACATTGCA ACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACA ATTTTCAACTTGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCC TACTATAGCCCTCATGGACATATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAA ATGGAAGTGTGGGATGTGAAAAACTACAAACTTATTTCTAAACCGGTGGCTTCTGATTCT ACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAACAGCTACATGTGCTCCCAGG TTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTTGCACAAGTAT GATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCT AAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAA TTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTA TCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTGCAAAGCAGGAA GCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGAGCACACCACGA AACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAATCAAGAACCTA AAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGAT TTGGAATTGGGTATTTAA >OriGene 5' read for NM_032025 unedited
ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGACAACATGGCG CCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAATGGACCA CCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAAGTCTGT ATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAATATTATC AGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGCCTTGAA TTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACAGTACTTCTAAAGATGGC ACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTGAAATCT TTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACTCTTTGT GCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACAATTGCA AATAAATTGCATTTGCAAAAAATTAATGATTTTGTATTATCACCTGGACCCCAACCATAC AAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTATATCAG TACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAGGCAGAT AAAGTTACAATGCTGTGGAATAAAAAGCTACTGCTGTGTTGGGTATAGCTAGCACAGATG TTGACAGACAGGAGCTTCCTACTTGGAGAACANACTCTACACTACATTGCACAATGGNAG AAGTGGCTGTAGTGCCAATACCAAAAATGGCCCATTTATGGATGTAGTT >Forward primer walk for NM_032025 unedited
CTTTTTGTGGATTTTCCGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCA ATGAGGAACCTAAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCA CCAATTCCAAATTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACG ATAAGCCATTATCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTG CAAAGCAGGAAGCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGA GCACACCACGAAACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAA TCAAGAACCTAAAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTG GAAAACAGCTAGAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGG AGCTGGAAGATTTGGAATTGGGTATTTAAAGATTCACGGAAAGCAAGTTGATGACCAGAA ATCAGTGCAAACACATCTTCTGTTAAACCCATTGGTATACACAGAATATTCCTGTGCCCA CACTTAATGTCAATCTATAATTTTAACCATTTATCCAAGATTCTACTAAGTGTAAAATTA TTTAATAATGTCTATTAAATTGATATTTATATCTTGCATCCTATATCATGTCAATATGTG ATATAGAAAAGAGATACGTGAATTTTTTAGCTAAGCTTGACAGATTGAAAGACAAGTGTC ATTTTTTTTTGTAGAGGGTGATATATACCATGTAAATGAATAAAGACATTTTAAATTTAC AACAAAACAAAAACACTCGACTTCTAGAATTGCGCCCGCGGTCAATAGCTTGTTTCCTGA ACAAAATCCCGGGTGGCATCCCTGGTGACCCCTCCCCAGTGGCTCTTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032025 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032025.3, NP_114414.2 |
| RefSeq Size | 3894 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 83939 |
| UniProt ID | Q9BY44 |
| Gene Summary | This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204303 | EIF2A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A) |
CNY 4,360.00 |
|
| RC204303L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC204303L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), mGFP tagged |
CNY 6,460.00 |
|
| RC204303L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC204303L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A), mGFP tagged |
CNY 6,460.00 |
|
| RG204303 | EIF2A (tGFP-tagged) - Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A) |
CNY 5,040.00 |
