LTBP4 (NM_001042545) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARCL1C; LTBP-4; LTBP4L; LTBP4S
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216920 representing NM_001042545 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGGCGTGCGGCTGCTCTGGGTGTCGCTATTGGTGCTGCTGGCGCAGCTAGGGCCGCAGCCTG GACTGGGCCGGCTCGGAGAGCGTCTCCGCGTGCGCTTCACCCCGGTCGTGTGCGGCCTGCGCTGCGTCCA TGGGCCGACCGGCTCCCGCTGTACCCCGACCTGCGCGCCCCGCAACGCCACCAGCGTGGACAGCGGCGCT CCCGGCGGGGCGGCCCCGGGGGGACCCGGCTTCCGCGCCTTCCTGTGTCCCTTGATCTGTCACAATGGCG GTGTGTGCGTGAAGCCTGACCGCTGCCTCTGTCCCCCGGACTTCGCTGGCAAGTTCTGCCAGTTGCACTC CTCGGGCGCCCGGCCCCCGGCCCCGGCTGTACCAGGCCTCACCCGCTCCGTGTACACTATGCCACTGGCC AACCACCGCGACGACGAGCACGGCGTGGCATCTATGGTGAGCGTCCACGTGGAGCACCCGCAGGAGGCGT CGGTGGTGGTGCACCAGGTGGAGCGTGTGTCTGGCCCTTGGGAGGAGGCGGACGCTGAGGCGGTGGCGCG GGCGGAAGCGGCGGCGCGGGCGGAGGCGGCAGCGCCCTACACGGTGTTGGCACAGAGCGCGCCGCGGGAG GACGGCTACTCAGATGCCTCGGGCTTCGGTTACTGCTTTCGGGAGCTGCGCGGAGGCGAATGCGCGTCCC CGCTGCCCGGGCTCCGGACGCAGGAGGTCTGCTGCCGAGGGGCCGGCTTGGCCTGGGGCGTTCACGACTG TCAGCTGTGCTCCGAGCGCCTGGGGAACTCCGAAAGAGTGAGCGCCCCAGATGGACCTTGTCCAACCGGC TTTGAAAGAGTTAATGGGTCCTGCGAAGATGTGGATGAGTGCGCGACTGGCGGGCGCTGCCAGCACGGCG AGTGTGCAAACACGCGCGGCGGGTACACGTGTGTGTGCCCCGACGGCTTTCTGCTCGACTCGTCCCGCAG CAGCTGCATCTCCCAACACGTGATCTCAGAGGCCAAAGGGCCCTGCTTCCGCGTGCTCCGCGACGGCGGC TGTTCGCTGCCCATTCTGCGGAACATCACTAAACAGATCTGCTGCTGCAGCCGCGTAGGCAAGGCCTGGG GCCGGGGCTGCCAGCTCTGCCCACCCTTCGGCTCAGAGGGTTTCCGGGAGATCTGCCCGGCTGGTCCTGG TTACCACTACTCGGCCTCCGACCTCCGCTACAACACCAGACCCCTGGGCCAGGAGCCACCCCGAGTGTCA CTCAGCCAGCCTCGTACCCTGCCAGCCACCTCTCGGCCATCTGCAGGCTTTCTGCCCACCCATCGCCTGG AGCCCCGGCCTGAACCCCGGCCCGATCCCCGGCCCGGCCCTGAGCTTCCCTTGCCCAGCATCCCTGCCTG GACTGGTCCTGAGATTCCTGAATCAGGTCCCTCCTCCGGCATGTGTCAGCGCAACCCCCAGGTCTGCGGC CCAGGACGCTGCATTTCCCGGCCCAGCGGCTACACCTGCGCTTGCGACTCTGGCTTCCGGCTCAGCCCCC AGGGCACCCGATGCATTGATGTGGACGAATGTCGCCGCGTGCCCCCGCCCTGTGCTCCCGGGCGCTGCGA GAACTCACCAGGCAGCTTCCGCTGCGTGTGCGGCCCGGGCTTCCGAGCCGGCCCACGGGCTGCGGAATGC CTGGATGTGGACGAGTGCCACCGCGTGCCGCCGCCGTGTGACCTCGGGCGCTGCGAGAACACGCCAGGCA GCTTCCTGTGCGTGTGCCCCGCCGGGTACCAGGCTGCACCGCACGGAGCCAGCTGCCAGGATGTGGATGA ATGCACCCAGAGCCCAGGCCTGTGTGGCCGAGGGGCCTGCAAGAACCTGCCTGGCTCTTTCCGCTGTGTT TGCCCGGCTGGCTTCCGGGGCTCGGCGTGTGAAGAGGATGTGGATGAGTGTGCCCAGGAGCCGCCGCCCT GTGGGCCCGGCCGCTGTGACAACACGGCAGGCTCCTTTCACTGTGCCTGCCCTGCTGGCTTCCGCTCCCG AGGGCCCGGGGCCCCCTGCCAAGATGTGGATGAGTGTGCCCGAAGCCCCCCACCCTGCACCTACGGCCGG TGTGAGAACACAGAAGGCAGCTTCCAGTGTGTCTGCCCCATGGGCTTCCAACCCAACACTGCTGGCTCCG AGTGCGAGGATGTGGATGAGTGTGAGAACCACCTCGCATGCCCTGGGCAGGAGTGTGTGAACTCGCCCGG CTCCTTCCAGTGCAGGACCTGTCCTTCTGGCCACCACCTGCACCGTGGCAGATGCACTGATGTGGACGAA TGCAGTTCGGGTGCCCCTCCCTGTGGTCCCCACGGCCACTGCACTAACACCGAAGGCTCCTTCCGCTGCA GCTGCGCGCCAGGCTACCGGGCGCCGTCGGGTCGGCCCGGGCCCTGCGCAGACGTGAACGAGTGCCTGGA GGGCGATTTCTGCTTCCCTCACGGCGAGTGCCTCAACACTGACGGCTCCTTTGCCTGTACTTGTGCCCCT GGCTACCGACCCGGACCCCGCGGAGCCTCTTGCCTCGACGTTGACGAGTGCAGCGAGGAGGACCTTTGCC AGAGCGGCATCTGTACCAACACCGACGGCTCCTTCGAGTGCATCTGTCCTCCGGGACACCGCGCTGGCCC GGACCTCGCCTCCTGCCTCGACGTGGACGAATGTCGCGAGCGAGGCCCAGCCCTGTGCGGGTCGCAGCGC TGTGAGAACTCTCCCGGCTCCTACCGCTGTGTCCGGGACTGCGATCCTGGGTACCACGCGGGCCCCGAGG GCACCTGTGACGATGTGGATGAGTGCCAAGAATATGGTCCCGAGATTTGTGGAGCCCAGCGTTGTGAGAA CACCCCTGGCTCCTACCGCTGCACACCAGCCTGTGACCCTGGCTATCAGCCCACGCCAGGGGGCGGATGC CAGGATGTGGACGAATGCCGGAACCGGTCCTTCTGCGGTGCCCACGCCGTGTGCCAGAACCTGCCCGGCT CCTTCCAGTGCCTCTGTGACCAGGGTTACGAGGGGGCACGGGATGGGCGTCACTGCGTGGATGTGAACGA GTGTGAAACACTACAGGGTGTATGTGGAGCTGCCCTGTGTGAAAATGTCGAAGGCTCCTTCCTCTGTGTC TGCCCCAACAGCCCGGAAGAGTTTGACCCCATGACTGGACGCTGTGTTCCCCCACGAACTTCTGCTGGCA CGTTCCCAGGCTCGCAGCCCCAGGCACCTGCTAGCCCCGTTCTGCCCGCCAGGCCACCTCCGCCACCCCT GCCCCGCCGACCCAGCACACCTAGGCAGGGCCCTGTGGGGAGTGGGCGCCGGGAGTGCTACTTTGACACA GCGGCCCCGGATGCATGTGACAACATCCTGGCTCGGAATGTGACATGGCAGGAGTGCTGCTGTACTGTGG GTGAGGGCTGGGGCAGCGGCTGCCGCATCCAGCAGTGCCCGGGCACCGAGACAGCTGAGTACCAGTCATT GTGCCCTCACGGCCGGGGCTACCTGGCGCCCAGTGGAGACCTGAGCCTCCGGAGAGACGTGGACGAATGT CAGCTCTTCCGAGACCAGGTGTGCAAGAGTGGCGTGTGTGTGAACACGGCCCCGGGCTACTCATGCTATT GCAGCAACGGCTACTACTACCACACACAGCGGCTGGAGTGCATCGACAATGACGAGTGCGCCGATGAGGA ACCGGCCTGTGAGGGCGGCCGCTGTGTCAACACTGTGGGCTCTTATCACTGTACCTGCGAGCCCCCACTG GTGCTGGATGGCTCGCAGCGCCGCTGCGTCTCCAACGAGAGCCAGAGCCTCGATGACAATCTGGGAGTGT GCTGGCAGGAAGTGGGGGCTGACCTCGTGTGCAGCCACCCTCGGCTGGACCGTCAGGCCACCTACACAGA GTGCTGCTGCCTGTATGGAGAGGCCTGGGGCATGGACTGCGCCCTCTGCCCTGCGCAGGACTCAGATGAC TTCGAGGCCCTGTGCAATGTGCTACGCCCCCCCGCATATAGCCCCCCGCGACCAGGTGGCTTTGGACTCC CCTACGAGTACGGCCCAGACTTAGGTCCACCTTACCAGGGCCTCCCATATGGGCCTGAGTTGTACCCACC ACCTGCGCTACCCTACGACCCCTACCCACCGCCACCTGGGCCCTTCGCCCGCCGGGAGGCTCCTTATGGG GCACCCCGCTTCGACATGCCAGACTTTGAGGACGATGGTGGCCCCTATGGCGAATCTGAGGCTCCTGCGC CACCTGGCCCGGGCACCCGCTGGCCCTATCGGTCCCGGGACACCCGCCGCTCCTTCCCAGAGCCCGAGGA GCCTCCTGAAGGTGGAAGCTATGCTGGTTCCCTGGCTGAGCCCTACGAGGAGCTGGAGGCGGAGGAGTGC GGGATCCTGGACGGCTGCACCAACGGCCGCTGCGTGCGCGTCCCCGAAGGCTTCACCTGCCGTTGCTTCG ACGGCTACCGCCTGGACATGACCCGCATGGCCTGCGTTGACATCAACGAGTGTGATGAGGCCGAGGCTGC CTCCCCGCTGTGCGTCAACGCGCGTTGCCTCAACACGGATGGCTCCTTCCGCTGCATCTGCCGCCCGGGA TTCGCACCCACGCACCAGCCGCACCACTGTGCGCCCGCACGGCCCCGGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216920 representing NM_001042545 Red=Cloning site Green=Tags(s) MAGGVRLLWVSLLVLLAQLGPQPGLGRLGERLRVRFTPVVCGLRCVHGPTGSRCTPTCAPRNATSVDSGA PGGAAPGGPGFRAFLCPLICHNGGVCVKPDRCLCPPDFAGKFCQLHSSGARPPAPAVPGLTRSVYTMPLA NHRDDEHGVASMVSVHVEHPQEASVVVHQVERVSGPWEEADAEAVARAEAAARAEAAAPYTVLAQSAPRE DGYSDASGFGYCFRELRGGECASPLPGLRTQEVCCRGAGLAWGVHDCQLCSERLGNSERVSAPDGPCPTG FERVNGSCEDVDECATGGRCQHGECANTRGGYTCVCPDGFLLDSSRSSCISQHVISEAKGPCFRVLRDGG CSLPILRNITKQICCCSRVGKAWGRGCQLCPPFGSEGFREICPAGPGYHYSASDLRYNTRPLGQEPPRVS LSQPRTLPATSRPSAGFLPTHRLEPRPEPRPDPRPGPELPLPSIPAWTGPEIPESGPSSGMCQRNPQVCG PGRCISRPSGYTCACDSGFRLSPQGTRCIDVDECRRVPPPCAPGRCENSPGSFRCVCGPGFRAGPRAAEC LDVDECHRVPPPCDLGRCENTPGSFLCVCPAGYQAAPHGASCQDVDECTQSPGLCGRGACKNLPGSFRCV CPAGFRGSACEEDVDECAQEPPPCGPGRCDNTAGSFHCACPAGFRSRGPGAPCQDVDECARSPPPCTYGR CENTEGSFQCVCPMGFQPNTAGSECEDVDECENHLACPGQECVNSPGSFQCRTCPSGHHLHRGRCTDVDE CSSGAPPCGPHGHCTNTEGSFRCSCAPGYRAPSGRPGPCADVNECLEGDFCFPHGECLNTDGSFACTCAP GYRPGPRGASCLDVDECSEEDLCQSGICTNTDGSFECICPPGHRAGPDLASCLDVDECRERGPALCGSQR CENSPGSYRCVRDCDPGYHAGPEGTCDDVDECQEYGPEICGAQRCENTPGSYRCTPACDPGYQPTPGGGC QDVDECRNRSFCGAHAVCQNLPGSFQCLCDQGYEGARDGRHCVDVNECETLQGVCGAALCENVEGSFLCV CPNSPEEFDPMTGRCVPPRTSAGTFPGSQPQAPASPVLPARPPPPPLPRRPSTPRQGPVGSGRRECYFDT AAPDACDNILARNVTWQECCCTVGEGWGSGCRIQQCPGTETAEYQSLCPHGRGYLAPSGDLSLRRDVDEC QLFRDQVCKSGVCVNTAPGYSCYCSNGYYYHTQRLECIDNDECADEEPACEGGRCVNTVGSYHCTCEPPL VLDGSQRRCVSNESQSLDDNLGVCWQEVGADLVCSHPRLDRQATYTECCCLYGEAWGMDCALCPAQDSDD FEALCNVLRPPAYSPPRPGGFGLPYEYGPDLGPPYQGLPYGPELYPPPALPYDPYPPPPGPFARREAPYG APRFDMPDFEDDGGPYGESEAPAPPGPGTRWPYRSRDTRRSFPEPEEPPEGGSYAGSLAEPYEELEAEEC GILDGCTNGRCVRVPEGFTCRCFDGYRLDMTRMACVDINECDEAEAASPLCVNARCLNTDGSFRCICRPG FAPTHQPHHCAPARPRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001042545
ORF Size	4671 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001042545.2
RefSeq Size	4979 bp
RefSeq ORF	4674 bp
Locus ID	8425
UniProt ID	Q8N2S1
Protein Families	Druggable Genome
MW	165.6 kDa
Gene Summary	The protein encoded by this gene binds transforming growth factor beta (TGFB) as it is secreted and targeted to the extracellular matrix. TGFB is biologically latent after secretion and insertion into the extracellular matrix, and sheds TGFB and other proteins upon activation. Defects in this gene may be a cause of cutis laxa and severe pulmonary, gastrointestinal, and urinary abnormalities. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

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