LTBP4 (NM_001042545) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARCL1C; LTBP-4; LTBP4L; LTBP4S
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC311429 sequence for NM_001042545 edited (data generated by NextGen Sequencing) ATGGCGGGCGGCGTGCGGCTGCTCTGGGTGTCGCTATTGGTGCTGCTGGCGCAGCTAGGGCCGCAGCCTG GACTGGGCCGGCTCGGAGAGCGTCTCCGCGTGCGCTTCACCCCGGTCGTGTGCGGCCTGCGCTGCGTCCA TGGGCCGACCGGCTCCCGCTGTACCCCGACCTGCGCGCCCCGCAACGCCACCAGCGTGGACAGCGGCGCT CCCGGCGGGGCGGCCCCGGGGGGACCCGGCTTCCGCGCCTTCCTGTGTCCCTTGATCTGTCACAATGGCG GTGTGTGCGTGAAGCCTGACCGCTGCCTCTGTCCCCCGGACTTCGCTGGCAAGTTCTGCCAGTTGCACTC CTCGGGCGCCCGGCCCCCGGCCCCGGCTGTACCAGGCCTCACCCGCTCCGTGTACACTATGCCACTGGCC AACCACCGCGACGACGAGCACGGCGTGGCATCTATGGTGAGCGTCCACGTGGAGCACCCGCAGGAGGCGT CGGTGGTGGTGCACCAGGTGGAGCGTGTGTCTGGCCCTTGGGAGGAGGCGGACGCTGAGGCGGTGGCGCG GGCGGAAGCGGCGGCGCGGGCGGAGGCGGCAGCGCCCTACACGGTGTTGGCACAGAGCGCGCCGCGGGAG GACGGCTACTCAGATGCCTCGGGCTTCGGTTACTGCTTTCGGGAGCTGCGCGGAGGCGAATGCGCGTCCC CGCTGCCCGGGCTCCGGACGCAGGAGGTCTGCTGCCGAGGGGCCGGCTTGGCCTGGGGCGTTCACGACTG TCAGCTGTGCTCCGAGCGCCTGGGGAACTCCGAAAGAGTGAGCGCCCCAGATGGACCTTGTCCAACCGGC TTTGAAAGAGTTAATGGGTCCTGCGAAGATGTGGATGAGTGCGCGACTGGCGGGCGCTGCCAGCACGGCG AGTGTGCAAACACGCGCGGCGGGTACACGTGTGTGTGCCCCGACGGCTTTCTGCTCGACTCGTCCCGCAG CAGCTGCATCTCCCAACACGTGATCTCAGAGGCCAAAGGGCCCTGCTTCCGCGTGCTCCGCGACGGCGGC TGTTCGCTGCCCATTCTGCGGAACATCACTAAACAGATCTGCTGCTGCAGCCGCGTAGGCAAGGCCTGGG GCCGGGGCTGCCAGCTCTGCCCACCCTTCGGCTCAGAGGGTTTCCGGGAGATCTGCCCGGCTGGTCCTGG TTACCACTACTCGGCCTCCGACCTCCGCTACAACACCAGACCCCTGGGCCAGGAGCCACCCCGAGTGTCA CTCAGCCAGCCTCGTACCCTGCCAGCCACCTCTCGGCCATCTGCAGGCTTTCTGCCCACCCATCGCCTGG AGCCCCGGCCTGAACCCCGGCCCGATCCCCGGCCCGGCCCTGAGCTTCCCTTGCCCAGCATCCCTGCCTG GACTGGTCCTGAGATTCCTGAATCAGGTCCCTCCTCCGGCATGTGTCAGCGCAACCCCCAGGTCTGCGGC CCAGGACGCTGCATTTCCCGGCCCAGCGGCTACACCTGCGCTTGCGACTCTGGCTTCCGGCTCAGCCCCC AGGGCACCCGATGCATTGATGTGGACGAATGTCGCCGCGTGCCCCCGCCCTGTGCTCCCGGGCGCTGCGA GAACTCACCAGGCAGCTTCCGCTGCGTGTGCGGCCCGGGCTTCCGAGCCGGCCCACGGGCTGCGGAATGC CTGGATGTGGACGAGTGCCACCGCGTGCCGCCGCCGTGTGACCTCGGGCGCTGCGAGAACACGCCAGGCA GCTTCCTGTGCGTGTGCCCCGCCGGGTACCAGGCTGCACCGCACGGAGCCAGCTGCCAGGATGTGGATGA ATGCACCCAGAGCCCAGGCCTGTGTGGCCGAGGGGCCTGCAAGAACCTGCCTGGCTCTTTCCGCTGTGTT TGCCCGGCTGGCTTCCGGGGCTCGGCGTGTGAAGAGGATGTGGATGAGTGTGCCCAGGAGCCGCCGCCCT GTGGGCCCGGCCGCTGTGACAACACGGCAGGCTCCTTTCACTGTGCCTGCCCTGCTGGCTTCCGCTCCCG AGGGCCCGGGGCCCCCTGCCAAGATGTGGATGAGTGTGCCCGAAGCCCCCCACCCTGCACCTACGGCCGG TGTGAGAACACAGAAGGCAGCTTCCAGTGTGTCTGCCCCATGGGCTTCCAACCCAACACTGCTGGCTCCG AGTGCGAGGATGTGGATGAGTGTGAGAACCACCTCGCATGCCCTGGGCAGGAGTGTGTGAACTCGCCCGG CTCCTTCCAGTGCAGGACCTGTCCTTCTGGCCACCACCTGCACCGTGGCAGATGCACTGATGTGGACGAA TGCAGTTCGGGTGCCCCTCCCTGTGGTCCCCACGGCCACTGCACTAACACCGAAGGCTCCTTCCGCTGCA GCTGCGCGCCAGGCTACCGGGCGCCGTCGGGTCGGCCCGGGCCCTGCGCAGACGTGAACGAGTGCCTGGA GGGCGATTTCTGCTTCCCTCACGGCGAGTGCCTCAACACTGACGGCTCCTTTGCCTGTACTTGTGCCCCT GGCTACCGACCCGGACCCCGCGGAGCCTCTTGCCTCGACGTTGACGAGTGCAGCGAGGAGGACCTTTGCC AGAGCGGCATCTGTACCAACACCGACGGCTCCTTCGAGTGCATCTGTCCTCCGGGACACCGCGCTGGCCC GGACCTCGCCTCCTGCCTCGACGTGGACGAATGTCGCGAGCGAGGCCCAGCCCTGTGCGGGTCGCAGCGC TGTGAGAACTCTCCCGGCTCCTACCGCTGTGTCCGGGACTGCGATCCTGGGTACCACGCGGGCCCCGAGG GCACCTGTGACGATGTGGATGAGTGCCAAGAATATGGTCCCGAGATTTGTGGAGCCCAGCGTTGTGAGAA CACCCCTGGCTCCTACCGCTGCACACCAGCCTGTGACCCTGGCTATCAGCCCACGCCAGGGGGCGGATGC CAGGATGTGGACGAATGCCGGAACCGGTCCTTCTGCGGTGCCCACGCCGTGTGCCAGAACCTGCCCGGCT CCTTCCAGTGCCTCTGTGACCAGGGTTACGAGGGGGCACGGGATGGGCGTCACTGCGTGGATGTGAACGA GTGTGAAACACTACAGGGTGTATGTGGAGCTGCCCTGTGTGAAAATGTCGAAGGCTCCTTCCTCTGTGTC TGCCCCAACAGCCCGGAAGAGTTTGACCCCATGACTGGACGCTGTGTTCCCCCACGAACTTCTGCTGGCA CGTTCCCAGGCTCGCAGCCCCAGGCACCTGCTAGCCCCGTTCTGCCCGCCAGGCCACCTCCGCCACCCCT GCCCCGCCGACCCAGCACACCTAGGCAGGGCCCTGTGGGGAGTGGGCGCCGGGAGTGCTACTTTGACACA GCGGCCCCGGATGCATGTGACAACATCCTGGCTCGGAATGTGACATGGCAGGAGTGCTGCTGTACTGTGG GTGAGGGCTGGGGCAGCGGCTGCCGCATCCAGCAGTGCCCGGGCACCGAGACAGCTGAGTACCAGTCATT GTGCCCTCACGGCCGGGGCTACCTGGCGCCCAGTGGAGACCTGAGCCTCCGGAGAGACGTGGACGAATGT CAGCTCTTCCGAGACCAGGTGTGCAAGAGTGGCGTGTGTGTGAACACGGCCCCGGGCTACTCATGCTATT GCAGCAACGGCTACTACTACCACACACAGCGGCTGGAGTGCATCGACAATGACGAGTGCGCCGATGAGGA ACCGGCCTGTGAGGGCGGCCGCTGTGTCAACACTGTGGGCTCTTATCACTGTACCTGCGAGCCCCCACTG GTGCTGGATGGCTCGCAGCGCCGCTGCGTCTCCAACGAGAGCCAGAGCCTCGATGACAATCTGGGAGTGT GCTGGCAGGAAGTGGGGGCTGACCTCGTGTGCAGCCACCCTCGGCTGGACCGTCAGGCCACCTACACAGA GTGCTGCTGCCTGTATGGAGAGGCCTGGGGCATGGACTGCGCCCTCTGCCCTGCGCAGGACTCAGATGAC TTCGAGGCCCTGTGCAATGTGCTACGCCCCCCCGCATATAGCCCCCCGCGACCAGGTGGCTTTGGACTCC CCTACGAGTACGGCCCAGACTTAGGTCCACCTTACCAGGGCCTCCCATATGGGCCTGAGTTGTACCCACC ACCTGCGCTACCCTACGACCCCTACCCACCGCCACCTGGGCCCTTCGCCCGCCGGGAGGCTCCTTATGGG GCACCCCGCTTCGACATGCCAGACTTTGAGGACGATGGTGGCCCCTATGGCGAATCTGAGGCTCCTGCGC CACCTGGCCCGGGCACCCGCTGGCCCTATCGGTCCCGGGACACCCGCCGCTCCTTCCCAGAGCCCGAGGA GCCTCCTGAAGGTGGAAGCTATGCTGGTTCCCTGGCTGAGCCCTACGAGGAGCTGGAGGCGGAGGAGTGC GGGATCCTGGACGGCTGCACCAACGGCCGCTGCGTGCGCGTCCCCGAAGGCTTCACCTGCCGTTGCTTCG ACGGCTACCGCCTGGACATGACCCGCATGGCCTGCGTTGACATCAACGAGTGTGATGAGGCCGAGGCTGC CTCCCCGCTGTGCGTCAACGCGCGTTGCCTCAACACGGATGGCTCCTTCCGCTGCATCTGCCGCCCGGGA TTCGCACCCACGCACCAGCCGCACCACTGTGCGCCCGCACGGCCCCGGGCCTGA Clone variation with respect to NM_001042545.1
Restriction Sites	Please inquire
ACCN	NM_001042545
Insert Size	5000 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001042545.1, NP_001036010.1
RefSeq Size	4979 bp
RefSeq ORF	4674 bp
Locus ID	8425
UniProt ID	Q8N2S1
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene binds transforming growth factor beta (TGFB) as it is secreted and targeted to the extracellular matrix. TGFB is biologically latent after secretion and insertion into the extracellular matrix, and sheds TGFB and other proteins upon activation. Defects in this gene may be a cause of cutis laxa and severe pulmonary, gastrointestinal, and urinary abnormalities. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) has a shorter and distinct N-terminus compared to isoform a.

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