CPEB2 (NM_182646) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CPE-BP2; CPEB-2; hCPEB-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC217504 representing NM_182646 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGATTTCGGGTTTGGGGTGCTGCAGACCGCCCCGCTCCGAAGTAGCAGTCCTGGGCCCCTGTTCT GCGGCGAGGCGTATGGTCCTTACGCCGTGGGGTCCGTCAACCCGCTGCCCTCCGCCACGCCCTTCGGCCC ACTGTCGCCACCACCGTTGCCTGTCACCGGCTTCTTAGAGGCCGCCTCCCCCTTCTCCGTCCCCCTCGGC GGCGGCGCGGGCAGCCCGGCCGCCGCCGCTTCCTCTTCCTCCCCGTTCCTGGCGCATCAGCAGACCATGC AGGATGAGCTGCTTCTGGGGCTGACACAGCAGCCGGCGCGGCCGCTTTCGGGGGCGGCGGCCACGGAGAA ACTCCCCGACCACCACCCCGGCGGCGGCACGATCGCGGGTGTGACCCACCTCCTCCCCTCCCAGGACTTC AAACCGAGTCTGCACCACCCCTCCTCCTCCTCCGCCTCCTCCTGCTGCTGCTGCCGCACCTCCTCCCCGC AGGACTTCAGTAAGCGGCAGCAGCAGCAGCTGAGCAGCCAGAAGAGGAAAGAGTTCAGCCCTCCCCACCT TCCCCACCCTCCGGACTCGAAGCCGCCGCCGCCGCCTCCGCCGCTCCACTGCCCCGGTCGGTTCAGCCCG CCGCCGCCGCCAGCCGGCCCGCTCCTCCAGCCGGCGCAGCTCGCTCAGCGCCAGCAGCAACAGCCGCCGC AGCAGTTCAGCCTCCTGCATCAGCAGCACCTCTCGCCGCAGGACTTCGCCCCGCGGCAGCGTCCGGCAGA CCTGCCCCCGCTCCCGCAGCTCCCTCCCTCGCCGCCTGCAGCCCCGCGGCGCCGCCACGGAGGCGCGGGC AGCCCTCGCAAGACCCCAGCCGCGGGCGAGGGCAGCGCCGCCGAGTCCCCCAATGCGGGCTTGGCCTCCT CGACGCCGGTGAACCCCGCGCCGGGCTCCATGGAGTCCCCCAACCACCCTCTGCTCAACAGTCCCAGTAA CCTCCTGCCCGGAGGTGCGCTTGGCGCGGGCGCCTTCAGCAGCCTGCAGAGCCCGGACCTTCCACACCCG GGCGGCGGCGGCGGCGGCGGGGGCGGGGGGCCCCCAGGAGGCGGAGGGGGAGGCGGCTCCGCGTCGCCGC CGCCGCTGCCCGGCTTCGGCACCCCCTGGTCGGTGCAGACCGCGTCGCCGCCACCCCAGCCCCAGCAGCC GCCGCCGACCCAGCCGCAGCAGCAGCCGCCGCCACCCCAGCAGCCGCCCCAGCCGCAGCCGCAGCCGCCC GGCTCGTCTGCCACCACCCCGGGCGGCGGCAGCGGCGGCTCGCTCAGCGCCATGCCGCCGCCCAGCCCCG ACTCAGAGAACGGCTTCTACCCCGGGCTGCCGTCGTCCATGAACCCGGCCTTCTTCCCTAGCTTCTCGCC CGTGTCGCCGCACGGCTGCACTGGGCTCAGCGTTCCGACGAGCGGCGGCGGCGGCGGCGGCTTCGGCGGC CCCTTCTCGGCTACCGCTGTGCCCCCTCCGCCGCCGCCCGCCATGAATATACCTCAACAGCAGCCCCCGC CGCCCGCGGCGCCGCAGCAGCCGCAGAGCCGGAGGTCGCCCGTCAGCCCGCAGCTCCAGCAGCAGCACCA GGCGGCGGCCGCCGCCTTCCTGCAGCAGAGGAACTCCTATAACCACCACCAGCCTCTTCTGAAACAGTCT CCCTGGAGCAACCATCAGAGCAGTGGCTGGGGCACTGGAAGTATGTCCTGGGGAGCAATGCATGGCAGAG ATCATCGTAGAACCGGAAACATGGGAATCCCAGGAACTATGAATCAGATATCTCCATTGAAGAAACCGTT TTCTGGTAATGTCATAGCACCACCGAAATTTACTCGCTCAACTCCATCACTGACTCCAAAATCTTGGATT GAAGATAATGTGTTCAGAACAGACAACAATAGTAATACACTCTTACCCTTACAGGATCGAAGTAGAATGT ATGACAGTTTGAATATGCACTCTTTGGAAAATTCCCTTATCGATATTATGAGAGCAGAGCATGATCCTCT TAAGGGTCGATTGAGCTATCCACATCCAGGAACTGACAATCTGTTGATGTTAAATGGTCGTTCTTCCCTC TTTCCAATAGATGATGGCTTGCTTGATGATGGTCACAGTGATCAAGTTGGAGTTTTAAATTCACCCACCT GTTATTCAGCTCACCAAAATGGAGAGCGAATAGAACGCTTCTCTCGAAAAGTTTTTGTTGGTGGTCTTCC TCCAGATATTGATGAAGATGAAATAACTGCTAGCTTCAGAAGATTTGGGCCTTTGGTAGTAGATTGGCCT CATAAAGCAGAAAGCAAGTCCTATTTTCCACCAAAAGGCTATGCATTTCTTCTCTTTCAAGAAGAGAGCT CAGTTCAGGCACTCATTGATGCTTGTATTGAAGAAGATGGAAAACTCTATCTGTGTGTTTCTAGCCCTAC TATCAAGGACAAACCAGTTCAAATACGTCCTTGGAATTTAAGTGATAGTGATTTTGTAATGGATGGTTCT CAGCCTTTGGATCCCCGAAAAACAATTTTTGTTGGAGGTGTTCCTAGGCCATTAAGGGCTGTGGAACTTG CTATGATCATGGACCGGCTGTATGGTGGAGTTTGTTATGCAGGAATTGATACAGATCCTGAGCTAAAATA CCCAAAAGGTGCTGGGCGAGTTGCTTTCTCCAATCAGCAGAGCTATATTGCTGCCATTAGTGCTCGGTTT GTTCAGCTTCAGCATGGTGATATTGATAAACGTGTGGAGGTAAAGCCATATGTGCTAGATGACCAGATGT GTGATGAATGCCAGGGCGCACGCTGTGGTGGAAAATTTGCTCCCTTTTTTTGTGCCAATGTCACTTGCCT GCAGTATTACTGTGAGTTTTGTTGGGCAAATATCCACTCTCGTGCTGGACGTGAGTTCCATAAGCCATTG GTAAAGGAAGGTGCTGATCGCCCACGTCAGATCCACTTCCGCTGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC217504 representing NM_182646 Red=Cloning site Green=Tags(s) MRDFGFGVLQTAPLRSSSPGPLFCGEAYGPYAVGSVNPLPSATPFGPLSPPPLPVTGFLEAASPFSVPLG GGAGSPAAAASSSSPFLAHQQTMQDELLLGLTQQPARPLSGAAATEKLPDHHPGGGTIAGVTHLLPSQDF KPSLHHPSSSSASSCCCCRTSSPQDFSKRQQQQLSSQKRKEFSPPHLPHPPDSKPPPPPPPLHCPGRFSP PPPPAGPLLQPAQLAQRQQQQPPQQFSLLHQQHLSPQDFAPRQRPADLPPLPQLPPSPPAAPRRRHGGAG SPRKTPAAGEGSAAESPNAGLASSTPVNPAPGSMESPNHPLLNSPSNLLPGGALGAGAFSSLQSPDLPHP GGGGGGGGGGPPGGGGGGGSASPPPLPGFGTPWSVQTASPPPQPQQPPPTQPQQQPPPPQQPPQPQPQPP GSSATTPGGGSGGSLSAMPPPSPDSENGFYPGLPSSMNPAFFPSFSPVSPHGCTGLSVPTSGGGGGGFGG PFSATAVPPPPPPAMNIPQQQPPPPAAPQQPQSRRSPVSPQLQQQHQAAAAAFLQQRNSYNHHQPLLKQS PWSNHQSSGWGTGSMSWGAMHGRDHRRTGNMGIPGTMNQISPLKKPFSGNVIAPPKFTRSTPSLTPKSWI EDNVFRTDNNSNTLLPLQDRSRMYDSLNMHSLENSLIDIMRAEHDPLKGRLSYPHPGTDNLLMLNGRSSL FPIDDGLLDDGHSDQVGVLNSPTCYSAHQNGERIERFSRKVFVGGLPPDIDEDEITASFRRFGPLVVDWP HKAESKSYFPPKGYAFLLFQEESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRPWNLSDSDFVMDGS QPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARF VQLQHGDIDKRVEVKPYVLDDQMCDECQGARCGGKFAPFFCANVTCLQYYCEFCWANIHSRAGREFHKPL VKEGADRPRQIHFRWN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_182646
ORF Size	2988 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_182646.3
RefSeq Size	6764 bp
RefSeq ORF	2991 bp
Locus ID	132864
UniProt ID	Q7Z5Q1
MW	105.6 kDa
Gene Summary	The protein encoded by this gene is highly similar to cytoplasmic polyadenylation element binding protein (CPEB), an mRNA-binding protein that regulates cytoplasmic polyadenylation of mRNA as a trans factor in oogenesis and spermatogenesis. Studies of the similar gene in mice suggested a possible role of this protein in transcriptionally inactive haploid spermatids. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

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