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CPEB2 (NM_182646) Human Tagged ORF Clone
CAT＃: RG217504
- TrueORF®
CPEB2 (tGFP-tagged) - Human cytoplasmic polyadenylation element binding protein 2 (CPEB2), transcript variant A
ORF Plasmid: DDK
"NM_182646" in other vectors (2)
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CNY 10,740.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CPE-BP2; CPEB-2; hCPEB-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217504 representing NM_182646
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCGCCCAGCCCCGACTCAGAGAACGGCTTCTACCCCGGGCTGCCGTCGTCCATGAACCCGGCCT TCTTCCCTAGCTTCTCGCCCGTGTCGCCGCACGGCTGCACTGGGCTCAGCGTTCCGACGAGCGGCGGCGG CGGCGGCGGCTTCGGCGGCCCCTTCTCGGCTACCGCTGTGCCCCCTCCGCCGCCGCCCGCCATGAATATA CCTCAACAGCAGCCCCCGCCGCCCGCGGCGCCGCAGCAGCCGCAGAGCCGGAGGTCGCCCGTCAGCCCGC AGCTCCAGCAGCAGCACCAGGCGGCGGCCGCCGCCTTCCTGCAGCAGAGGAACTCCTATAACCACCACCA GCCTCTTCTGAAACAGTCTCCCTGGAGCAACCATCAGAGCAGTGGCTGGGGCACTGGAAGTATGTCCTGG GGAGCAATGCATGGCAGAGATCATCGTAGAACCGGAAACATGGGAATCCCAGGAACTATGAATCAGATAT CTCCATTGAAGAAACCGTTTTCTGGTAATGTCATAGCACCACCGAAATTTACTCGCTCAACTCCATCACT GACTCCAAAATCTTGGATTGAAGATAATGTGTTCAGAACAGACAACAATAGTAATACACTCTTACCCTTA CAGGATCGAAGTAGAATGTATGACAGTTTGAATATGCACTCTTTGGAAAATTCCCTTATCGATATTATGA GAGCAGAGCATGATCCTCTTAAGGGTCGATTGAGCTATCCACATCCAGGAACTGACAATCTGTTGATGTT AAATGGTCGTTCTTCCCTCTTTCCAATAGATGATGGCTTGCTTGATGATGGTCACAGTGATCAAGTTGGA GTTTTAAATTCACCCACCTGTTATTCAGCTCACCAAAATGGAGAGCGAATAGAACGCTTCTCTCGAAAAG TTTTTGTTGGTGGTCTTCCTCCAGATATTGATGAAGATGAAATAACTGCTAGCTTCAGAAGATTTGGGCC TTTGGTAGTAGATTGGCCTCATAAAGCAGAAAGCAAGTCCTATTTTCCACCAAAAGGCTATGCATTTCTT CTCTTTCAAGAAGAGAGCTCAGTTCAGGCACTCATTGATGCTTGTATTGAAGAAGATGGAAAACTCTATC TGTGTGTTTCTAGCCCTACTATCAAGGACAAACCAGTTCAAATACGTCCTTGGAATTTAAGTGATAGTGA TTTTGTAATGGATGGTTCTCAGCCTTTGGATCCCCGAAAAACAATTTTTGTTGGAGGTGTTCCTAGGCCA TTAAGGGCTGTGGAACTTGCTATGATCATGGACCGGCTGTATGGTGGAGTTTGTTATGCAGGAATTGATA CAGATCCTGAGCTAAAATACCCAAAAGGTGCTGGGCGAGTTGCTTTCTCCAATCAGCAGAGCTATATTGC TGCCATTAGTGCTCGGTTTGTTCAGCTTCAGCATGGTGATATTGATAAACGTGTGGAGGTAAAGCCATAT GTGCTAGATGACCAGATGTGTGATGAATGCCAGGGCGCACGCTGTGGTGGAAAATTTGCTCCCTTTTTTT GTGCCAATGTCACTTGCCTGCAGTATTACTGTGAGTTTTGTTGGGCAAATATCCACTCTCGTGCTGGACG TGAGTTCCATAAGCCATTGGTAAAGGAAGGTGCTGATCGCCCACGTCAGATCCACTTCCGCTGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217504 representing NM_182646
Red=Cloning site Green=Tags(s) MPPPSPDSENGFYPGLPSSMNPAFFPSFSPVSPHGCTGLSVPTSGGGGGGFGGPFSATAVPPPPPPAMNI PQQQPPPPAAPQQPQSRRSPVSPQLQQQHQAAAAAFLQQRNSYNHHQPLLKQSPWSNHQSSGWGTGSMSW GAMHGRDHRRTGNMGIPGTMNQISPLKKPFSGNVIAPPKFTRSTPSLTPKSWIEDNVFRTDNNSNTLLPL QDRSRMYDSLNMHSLENSLIDIMRAEHDPLKGRLSYPHPGTDNLLMLNGRSSLFPIDDGLLDDGHSDQVG VLNSPTCYSAHQNGERIERFSRKVFVGGLPPDIDEDEITASFRRFGPLVVDWPHKAESKSYFPPKGYAFL LFQEESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRP LRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHGDIDKRVEVKPY VLDDQMCDECQGARCGGKFAPFFCANVTCLQYYCEFCWANIHSRAGREFHKPLVKEGADRPRQIHFRWN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182646 |
| ORF Size | 1677 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182646.1, NP_872587.1 |
| RefSeq Size | 5537 bp |
| RefSeq ORF | 2991 bp |
| Locus ID | 132864 |
| UniProt ID | Q7Z5Q1 |
| Gene Summary | The protein encoded by this gene is highly similar to cytoplasmic polyadenylation element binding protein (CPEB), an mRNA-binding protein that regulates cytoplasmic polyadenylation of mRNA as a trans factor in oogenesis and spermatogenesis. Studies of the similar gene in mice suggested a possible role of this protein in transcriptionally inactive haploid spermatids. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
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