DNA Ligase I (LIG1) (NM_001289064) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239679 representing NM_001289064 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCGAAGTATCATGTCATTTTTCCACCCCAAGAAAGAGGGTAAAGCAAAGAAGCCTGAGAAGGAGG CATCCAATAGCAGCAGAGAGACGGAGCCCCCTCCAAAGGCGGCACTGAAGGAGTGGAATGGAGTGGTGTC CGAGAGTGACTCTCCGGTGAAGAGGCCAGGGAGGAAGGCGGCCCGGGTCCTGGGCAGCGAAGGGGAAGAG GAGGATGAAGCCCTTAGCCCTGCTAAAGGCCAGAAGCCTGCCCTGGACTGCTCACAGGTCTCCCCGCCCC GTCCTGCCACATCTCCTGAGAACAATGCTTCCCTCTCTGACACCTCTCCCATGGACAGTTCCCCATCAGG GATTCCGAAGCGTCGCACAGAAGCAGAGACCCCGACGGAAAGCGTTTCAGAGCCTGAGGTGGCCACGAAG CAGGAACTGCAGGAGGAGGAAGAGCAGACCAAGCCTCCCCGCAGAGCTCCCAAGACGCTCAGCAGCTTCT TCACCCCCCGGAAGCCAGCAGTCAAAAAAGAAGTGAAGGAAGAGGAGCCAGGGGCTCCAGGAAAGGAGGG AGCTGCTGAGGGACCCCTGGATCCATCTGGTTACAATCCTGCCAAGAACAACTATCATCCCGTGGAAGAT GCCTGCTGGAAACCGGGCCAGAAGGTTCCTTACCTGGCTGTGGCCCGGACGTTTGAGAAGATCGAGGAGG TGTCTGCTCGGCTCCGGATGGTGGAGACGCTGAGCAACTTGCTGCGCTCCGTGGTGGCCCTGTCGCCTCC AGACCTCCTCCCTGTCCTCTACCTCAGCCTCAACCACCTTGGGCCACCCCAGCAGGGCCTGGAGCTTGGC GTGGGTGATGGTGTCCTTCTCAAGGCAGTGGCCCAGGCCACAGGTCGGCAGCTGGAGTCCGTCCGGGCTG AGGCAGCCGAGAAAGGCGACGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACCCAGAGGCTCATGCTGCC ACCACCTCCGCTCACTGCCTCCGGGGTCTTCAGCAAGTTCCGCGACATCGCCAGGCTCACTGGCAGTGCT TCCACAGCCAAGAAGATAGACATCATCAAAGGCCTCTTTGTGGCCTGCCGCCACTCAGAAGCCCGGTTCA TCGCTAGGTCCCTGAGCGGACGGCTGCGCCTTGGGCTGGCAGAGCAGTCGGTGCTGGCTGCCCTCTCCCA GGCAGTGAGCCTCACGCCCCCGGGCCAAGAATTCCCACCAGCCATGGTGGATGCTGGGAAGGGCAAGACA GCAGAGGCCAGAAAGACGTGGCTGGAGGAGCAAGGCATGATCCTGAAGCAGACGTTCTGCGAGGTTCCCG ACCTGGACCGAATTATCCCCGTGCTGCTGGAGCACGGCCTGGAACGTCTCCCGGAGCACTGCAAGCTGAG CCCAGGGATTCCCCTGAAACCAATGTTGGCCCATCCCACCCGGGGCATCAGCGAGGTCCTGAAACGCTTT GAGGAGGCAGCTTTCACCTGCGAATACAAATATGACGGGCAGAGGGCACAGATCCACGCCCTGGAAGGCG GGGAGGTGAAGATCTTCAGCAGGAATCAGGAAGACAACACTGGGAAGTACCCGGACATCATCAGCCGCAT CCCCAAGATTAAACTCCCATCGGTCACATCCTTCATCCTGGACACCGAAGCCGTGGCTTGGGACCGGGAA AAGAAGCAGATCCAGCCATTCCAAGTGCTCACCACCCGCAAACGCAAGGAGGTGGATGCGTCTGAGATCC AGGTGCAGGTGTGTTTGTACGCCTTCGACCTCATCTACCTCAATGGAGAGTCCCTGGTACGTGAGCCCCT TTCCCGGCGCCGGCAGCTGCTCCGGGAGAACTTTGTGGAGACAGAGGGCGAGTTTGTCTTCGCCACCTCC CTGGACACCAAGGACATCGAGCAGATCGCCGAGTTCCTGGAGCAGTCAGTGAAAGACTCCTGCGAGGGGC TGATGGTGAAGACCCTGGATGTTGATGCCACCTACGAGATCGCCAAGAGATCGCACAACTGGCTCAAGCT GAAGAAGGACTACCTTGATGGCGTGGGTGACACCCTGGACCTGGTGGTGATCGGCGCCTACCTGGGCCGG GGGAAGCGGGCCGGCCGGTACGGGGGCTTCCTGCTGGCCTCCTACGACGAGGACAGTGAGGAGCTGCAGG CCATATGCAAGCTTGGAACTGGCTTCAGTGATGAGGAGCTGGAGGAGCATCACCAGAGCCTCAAGGCGCT GGTGCTGCCCAGCCCACGCCCTTACGTGCGGATAGATGGCGCTGTGATTCCCGACCACTGGCTGGACCCC AGCGCTGTGTGGGAGGTGAAGTGCGCTGACCTCTCCCTCTCTCCCATCTACCCTGCTGCGCGGGGCCTGG TGGATAGTGACAAGGGCATCTCCCTTCGCTTCCCTCGGTTTATTCGAGTCCGTGAAGACAAGCAGCCGGA GCAGGCCACCACCAGTGCTCAGGTGGCCTGTTTGTACCGGAAGCAAAGTCAGATTCAGAACCAACAAGGC GAGGACTCAGGCTCTGACCCTGAAGATACCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239679 representing NM_001289064 Red=Cloning site Green=Tags(s) MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEE EDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTEAETPTESVSEPEVATK QELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVED ACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELG VGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSA STAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKT AEARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRF EEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDRE KKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATS LDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGR GKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDP SAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQG EDSGSDPEDTY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289064
ORF Size	2553 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289064.1, NP_001275993.1
RefSeq Size	3206 bp
RefSeq ORF	2556 bp
Locus ID	3978
UniProt ID	P18858
Protein Families	Druggable Genome
Protein Pathways	Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair
MW	94.4 kDa
Gene Summary	This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.