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DNA Ligase I (LIG1) (NM_001289064) Human Tagged ORF Clone
CAT＃: RG239679
- TrueORF®
LIG1 (tGFP-tagged) - Human ligase I, DNA, ATP-dependent (LIG1), transcript variant 3
ORF Plasmid: DDK
"NM_001289064" in other vectors (2)
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CNY 7,510.00
货期*
2周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239679 representing NM_001289064.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGCGAAGTATCATGTCATTTTTCCACCCCAAGAAAGAGGGTAAAGCAAAGAAGCCTGAGAAGGAG GCATCCAATAGCAGCAGAGAGACGGAGCCCCCTCCAAAGGCGGCACTGAAGGAGTGGAATGGAGTGGTG TCCGAGAGTGACTCTCCGGTGAAGAGGCCAGGGAGGAAGGCGGCCCGGGTCCTGGGCAGCGAAGGGGAA GAGGAGGATGAAGCCCTTAGCCCTGCTAAAGGCCAGAAGCCTGCCCTGGACTGCTCACAGGTCTCCCCG CCCCGTCCTGCCACATCTCCTGAGAACAATGCTTCCCTCTCTGACACCTCTCCCATGGACAGTTCCCCA TCAGGGATTCCGAAGCGTCGCACAGAAGCAGAGACCCCGACGGAAAGCGTTTCAGAGCCTGAGGTGGCC ACGAAGCAGGAACTGCAGGAGGAGGAAGAGCAGACCAAGCCTCCCCGCAGAGCTCCCAAGACGCTCAGC AGCTTCTTCACCCCCCGGAAGCCAGCAGTCAAAAAAGAAGTGAAGGAAGAGGAGCCAGGGGCTCCAGGA AAGGAGGGAGCTGCTGAGGGACCCCTGGATCCATCTGGTTACAATCCTGCCAAGAACAACTATCATCCC GTGGAAGATGCCTGCTGGAAACCGGGCCAGAAGGTTCCTTACCTGGCTGTGGCCCGGACGTTTGAGAAG ATCGAGGAGGTGTCTGCTCGGCTCCGGATGGTGGAGACGCTGAGCAACTTGCTGCGCTCCGTGGTGGCC CTGTCGCCTCCAGACCTCCTCCCTGTCCTCTACCTCAGCCTCAACCACCTTGGGCCACCCCAGCAGGGC CTGGAGCTTGGCGTGGGTGATGGTGTCCTTCTCAAGGCAGTGGCCCAGGCCACAGGTCGGCAGCTGGAG TCCGTCCGGGCTGAGGCAGCCGAGAAAGGCGACGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACCCAG AGGCTCATGCTGCCACCACCTCCGCTCACTGCCTCCGGGGTCTTCAGCAAGTTCCGCGACATCGCCAGG CTCACTGGCAGTGCTTCCACAGCCAAGAAGATAGACATCATCAAAGGCCTCTTTGTGGCCTGCCGCCAC TCAGAAGCCCGGTTCATCGCTAGGTCCCTGAGCGGACGGCTGCGCCTTGGGCTGGCAGAGCAGTCGGTG CTGGCTGCCCTCTCCCAGGCAGTGAGCCTCACGCCCCCGGGCCAAGAATTCCCACCAGCCATGGTGGAT GCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGACGTGGCTGGAGGAGCAAGGCATGATCCTGAAGCAG ACGTTCTGCGAGGTTCCCGACCTGGACCGAATTATCCCCGTGCTGCTGGAGCACGGCCTGGAACGTCTC CCGGAGCACTGCAAGCTGAGCCCAGGGATTCCCCTGAAACCAATGTTGGCCCATCCCACCCGGGGCATC AGCGAGGTCCTGAAACGCTTTGAGGAGGCAGCTTTCACCTGCGAATACAAATATGACGGGCAGAGGGCA CAGATCCACGCCCTGGAAGGCGGGGAGGTGAAGATCTTCAGCAGGAATCAGGAAGACAACACTGGGAAG TACCCGGACATCATCAGCCGCATCCCCAAGATTAAACTCCCATCGGTCACATCCTTCATCCTGGACACC GAAGCCGTGGCTTGGGACCGGGAAAAGAAGCAGATCCAGCCATTCCAAGTGCTCACCACCCGCAAACGC AAGGAGGTGGATGCGTCTGAGATCCAGGTGCAGGTGTGTTTGTACGCCTTCGACCTCATCTACCTCAAT GGAGAGTCCCTGGTACGTGAGCCCCTTTCCCGGCGCCGGCAGCTGCTCCGGGAGAACTTTGTGGAGACA GAGGGCGAGTTTGTCTTCGCCACCTCCCTGGACACCAAGGACATCGAGCAGATCGCCGAGTTCCTGGAG CAGTCAGTGAAAGACTCCTGCGAGGGGCTGATGGTGAAGACCCTGGATGTTGATGCCACCTACGAGATC GCCAAGAGATCGCACAACTGGCTCAAGCTGAAGAAGGACTACCTTGATGGCGTGGGTGACACCCTGGAC CTGGTGGTGATCGGCGCCTACCTGGGCCGGGGGAAGCGGGCCGGCCGGTACGGGGGCTTCCTGCTGGCC TCCTACGACGAGGACAGTGAGGAGCTGCAGGCCATATGCAAGCTTGGAACTGGCTTCAGTGATGAGGAG CTGGAGGAGCATCACCAGAGCCTCAAGGCGCTGGTGCTGCCCAGCCCACGCCCTTACGTGCGGATAGAT GGCGCTGTGATTCCCGACCACTGGCTGGACCCCAGCGCTGTGTGGGAGGTGAAGTGCGCTGACCTCTCC CTCTCTCCCATCTACCCTGCTGCGCGGGGCCTGGTGGATAGTGACAAGGGCATCTCCCTTCGCTTCCCT CGGTTTATTCGAGTCCGTGAAGACAAGCAGCCGGAGCAGGCCACCACCAGTGCTCAGGTGGCCTGTTTG TACCGGAAGCAAAGTCAGATTCAGAACCAACAAGGCGAGGACTCAGGCTCTGACCCTGAAGATACCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239679
Blue=ORF Red=Cloning site Green=Tag(s) MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGE EEDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTEAETPTESVSEPEVA TKQELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHP VEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQG LELGVGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIAR LTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVD AGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGI SEVLKRFEEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDT EAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVET EGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLD LVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRID GAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACL YRKQSQIQNQQGEDSGSDPEDTY TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001289064 |
| ORF Size | 2553 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001289064.1, NP_001275993.1 |
| RefSeq Size | 3206 bp |
| RefSeq ORF | 2556 bp |
| Locus ID | 3978 |
| UniProt ID | P18858 |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair |
| MW | 94.4 kDa |
| Gene Summary | This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
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