VARS2 (NM_020442) Human Untagged Clone
CAT＃: SC100467
VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 3736.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COXPD20; VALRS; VARS2L; VARSL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_020442, the custom clone sequence may differ by one or more nucleotides
ATGCCTCATTTGCCTCTCGCCTCTTTTCGACCACCATTTTGGGGGCTGAGGCACTCACGGGGCCTCCCCA GGTTTCACTCCGTTTCTACACAGTCGGAGCCCCATGGATCTCCCATCTCCCGGAGGAACCGTGAAGCCAA ACAGAAGCGCCTGCGAGAGAAGCAGGCGACTCTGGAGGCTGAGATAGCAGGGGAGAGCAAGTCACCTGCA GAATCCATTAAGGCCTGGAGGCCTAAGGAGTTAGTATTGTATGAAATCCCTACGAAACCCGGTGAAAAGA AAGATGTCTCTGGGCCCCTGCCTCCTGCATACAGCCCCCGATATGTTGAGGCTGCCTGGTACCCGTGGTG GGTACGAGAGGGCTTCTTCAAACCAGAATATCAGGCCCGGCTGCCCCAAGCTACAGGGGAGACCTTTTCC ATGTGTATCCCACCTCCCAATGTCACTGGCTCCCTGCACATTGGCCACGCACTCACGGTGGCCATACAGG ATGCCCTCGTGCGCTGGCACCGGATGCGTGGGGATCAAGTGCTGTGGGTCCCTGGTTCAGATCATGCAGG AATTGCTACACAAGCTGTGGTGGAGAAACAACTGTGGAAGGAACGGGGAGTGAGGAGACATGAGCTGAGC CGGGAGGCCTTCCTTAGGGAGGTGTGGCAGTGGAAGGAGGCGAAAGGTGGAGAGATCTGTGAGCAGCTGC GAGCTCTGGGTGCCTCCCTGGACTGGGATCGAGAGTGTTTTACCATGGATGTTGGCTCCTCAGTGGCTGT GACTGAAGCTTTTGTGCGGCTCTACAAGGCGGGGTTGCTGTACCGGAACCATCAGCTTGTCAACTGGTCA TGTGCTTTAAGATCAGCCATCTCGGACATTGAGGTGGAGAACCGGCCCCTGCCTGGCCACACACAGCTTC GACTGCCTGGCTGCCCCACCCCCGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTCCCCGTGGATGGAGA GCCTGATGCAGAGGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGATGTGGCTGTGGCCGTT CATCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCCTTGATGGGGCAGCCTC TTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGTGAAGGTGACTCCAGCTCA CAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAGCCCCTTGAATGTCATTGCGGAGGATGGG ACCATGACCTCCCTCTGCGGGGACTGGCTGCAGGGTCTTCACCGGTTTGTGGCCCGGGAAAAGATAATGT CTGTGCTGAGTGAATGGGGCCTGTTCCGGGGCCTCCAGAACCACCCCATGGTACTGCCCATCTGCAGCCG TTCTGGGGATGTGATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGCTGCCAGGAAATGGGGGCCCGA GCTGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTCCACCAGAAGAACTGGCAGCACT GGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCATCAGATTCCAGCCTACCT GGTTGTAGAGGACCATGCGCAGGGAGAAGAGGACTGTTGGGTGGTTGGGCGGTCAGAGGCTGAGGCCAGA GAGGTAGCAGCGGAACTGACAGGGAGGCCAGGGGCAGAGCTGACCCTGGAGAGGGATCCTGATGTCCTAG ACACATGGTTTTCTTCTGCCCTGTTCCCCTTTTCTGCCCTGGGCTGGCCCCAAGAGACCCCAGACCTTGC TCGTTTCTACCCCCTGTCACTTTTGGAAACGGGCAGCGACCTTCTGCTGTTCTGGGTGGGCCGCATGGTC ATGTTGGGGACCCAGCTCACAGGGCAGCTGCCCTTCAGCAAGGTGCTTCTTCATCCCATGGTTCGGGACA GGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAATGTGCTGGACCCAAGAGACATCATCAGTGGGGTGGA GATGCAGGTGCTGCAGGAAAAGCTGAGAAGCGGAAATTTGGACCCTGCAGAGCTGGCCATTGTGGCTGCA GCACAGAAAAAGGACTTTCCTCACGGGATCCCTGAGTGTGGGACAGATGCCCTGAGATTCACACTCTGCT CCCATGGAGTTCAGGCGGGCGACTTGCACCTGTCAGTCTCTGAGGTCCAGAGCTGCCGACATTTCTGCAA CAAGATCTGGAATGCTCTTCGCTTTATCCTCAATGCTTTAGGGGAGAAATTTGTGCCACAGCCTGCTGAG GAGCTGTCTCCCTCCTCCCCGATGGATGCCTGGATCCTGAGCCGCCTTGCCCTGGCTGCCCAGGAGTGTG AGCGGGGCTTCCTCACCCGAGAGCTCTCGCTCGTCACTCATGCCCTGCACCACTTCTGGCTTCACAACCT CTGTGACGTCTACCTGGAGGCTGTGAAGCCCGTGCTGTGGCACTCGCCCCGCCCCCTGGGGCCCCCTCAG GTCCTGTTCTCCTGCGCTGACCTCGGCCTCCGCCTCCTGGCCCCACTGATGCCCTTCCTGGCTGAAGAGC TCTGGCAGAGGCTGCCCCCCAGGCCTGGTTGCCCCCCTGCCCCCAGCATCTCGGTTGCCCCCTACCCCAG CGCCTGCAGCTTGGAGCACTGGCGCCAGCCAGAGCTGGAGCGGCGCTTCTCCCGGGTCCAAGAGGTCGTG CAGGTGCTAAGGGCTCTCCGAGCCACGTACCAGCTCACCAAAGCCCGGCCCCGAGTGCTGCTGCAGAGCT CAGAGCCTGGGGACCAGGGCCTCTTCGAGGCCTTCTTGGAGCCCCTGGGCACCCTGGGCTACTGTGGGGC TGTGGGCCTGTTACCCCCAGGCGCAGCAGCTCCCTCCGGCTGGGCCCAGGCTCCACTCAGTGACACGGCT CAAGTCTACATGGAGCTGCAGGGCCTGGTGGACCCGCAGATCCAGCTACCTCTGTTAGCCGCCCGAAGGT ACAAGTTGCAGAAGCAGCTTGACAGCCTCACAGCCAGGACCCCATCAGAAGGGGAGGCAGGGACTCAGAG GCAACAAAAGCTTTCTTCCCTCCAGCTGGAATTGTCAAAACTGGACAAGGCAGCCTCTCACCTCCGGCAG CTGATGGATGAGCCTCCAGCCCCAGGGAGCCCGGAGCTCTAA >OriGene 5' read for NM_020442 unedited
GGTGTTCAAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACCCC CGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTCCCCGTGGATGGAGAGCCTGATGCAGA GGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGATGTGGCTGTGGCCGTTCA TCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCCTTGATGGG GCAGCCTCTTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGT GAAGGTGACTCCAGCTCACAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAG CCCCTTGAATGTCATTGCGGAGGATGGGACCATGACCTCTCTCTGCGGGGACTGGCTGCA GGGTCTTCACCGGTTTGTGGCCCGGGAAAAGATAATGTCTGTGCTGAGTGAACGGGGCCT GTTCCGGGGCCTCCAGAACCACCCCATGGTACTGCCCATCTGCAGCCGTTCTGGGGATGT GATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGCTGCCAGGAAATGGGGGCCCGAGC TGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTCCACCAGAAGAACTG GCAGCACTGGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCA TCAGATTCCAGCCTACCTGGTTGTAGAAGACCATGCGCAGGGAGAGAAGACTGTTGGGTG GTCGGGCGGTCAAAGGCTGATGCCANATAAGTANCANCNGAACTGACAGGGGAGCCAAGG GCANAGCTGACCCTGNAAAGGGATTCTGATGTNCTAAACACATTGTTTCTTCTGGCCTGT TTCCTTTTTTTGCCTGGGCT >OriGene 3' read for NM_020442 unedited
TTCNANGATCGGATTTTTTTTTTTTTTTTTTGGGGATTCAAAATTTACTCATCTCCTCTT GGGGGACTTCCAGTGGGAGGCAAGCCGGGCAGTAATGTGAGCATCTCAAGGACACCACAG TCACTGCGACCAAGCCAGTCTGTGTCCTCATTTACACAATGAAGGCGATGGACCACATAG TCTCTGACGTCCCACTGCACCCTGACAGCTGACAAAGGGGGGGGTCCTCAAAAACAGGTC TGAGAGGGAGGAAAACTGATGGGGATGATGAGTTAAAGCTCCGGGCTCCCTGGGGCTGGA GGCTCATCCATCAACTGCCGGAGGTGAAAGGCTGCCTTGTCCAGTTTTGACAATTCCAGC TGGAGGGAAAAAAGCTTTTGTTGCCTCTGAGTCCCTGCCTCCCCTTCTGATGGGGTCCTG GCTGTGAGGCTATCAAGCTGCTTCTGCAACTTGTACCTTCGGGCGGCTAACAGAGGTAGC TGGATCTGCGGGTCCACCAGGCCCTGCAGCTCCATGTAGACTTGAGCCGTGTCACTGAGT GGAGCCTGGGCCCAGCCGGAGGGAGCTGCTGCGCCTGGGGGTAACAGGCCCACAGCCCCA CAGTAGCCCAGGGTGCCCAGGGGCTCCAAGAAGGCCTCGAAGAGGCCCTGGTCCCCAAGC TCTGAGCTCTGCAGCANCACTCGGGGCCGGGCTTTGGTGAGCTGGTACCTGGCTTGGAGA GCCCTTAGCACCTGGACGACCTCTTGGGACCCGGGAGAAGCGCCGCTCCCACCTCTGGCT GGCCCCCTGGCTCCAAGCTGGCAGCGCTGGGGTAGGGGGGAACCGGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020442 |
| Insert Size | 2750 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020442.2, NP_065175.2 |
| RefSeq Size | 3625 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 57176 |
| UniProt ID | Q5ST30 |
| Domains | tRNA-synt_1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis |
| Gene Summary | This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (2) contains alternate 5' exon structure, and it thus differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211310 | VARS2 (Myc-DDK-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7400.00 |
|
| RC211310L3 | Lenti ORF clone of Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 12540.00 |
|
| RC211310L4 | Lenti ORF clone of Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 12540.00 |
|
| RG211310 | VARS2 (tGFP-tagged) - Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9000.00 |
|
| SC318474 | VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7408.00 |
